A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays.

A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays. Research Abstract Details 

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A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays. Abstract Text:

Nazila Salamat-Miller,Jianwen Fang,Christopher W Seidel,Aaron M Smalter,Yassen Assenov,Mario Albrecht,C Russell Middaugh,

The high affinity of certain cellular polyanions for many proteins (polyanion-binding proteins (PABPs)) has been demonstrated previously. It has been hypothesized that such polyanions may be involved in protein structure stabilization, stimulation of folding through chaperone-like activity, and intra- and extracellular protein transport as well as intracellular organization. The purpose of the proteomics studies reported here was to seek evidence for the idea that the nonspecific but high affinity interactions of PABPs with polyanions have a functional role in intracellular processes. Utilizing yeast protein arrays and five biotinylated cellular polyanion probes (actin, tubulin, heparin, heparan sulfate, and DNA), we identified proteins that interact with these probes and analyzed their structural and amino acid sequence requirements as well as their predicted functions in the yeast proteome. We also provide evidence for the existence of a network-like system for PABPs and their potential roles as critical hubs in intracellular behavior. This investigation takes a first step toward achieving a better understanding of the nature of polyanion-protein interactions within cells and introduces an alternative way of thinking about intracellular organization.

A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays. Publishing Authors By Initials

N Salamat-Miller,J Fang,CW Seidel,AM Smalter,Y Assenov,M Albrecht,CR Middaugh,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

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A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular & cellular proteomics : MCP

VOLUME: 5

Page Numbers: 2263-78

Journal Abbreviation: Mol. Cell Proteomics

ISSN: 1535-9476

DAY: 18

MONTH: 09

YEAR: 2006

A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101125647

A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: chemistry

Chemical & Substance for Abstract: A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays. Information

Substance Name: Heparitin Sulfate

Registry Number: 9050-30-0

Grant and Affiliation Information for A network-based analysis of polyanion-binding proteins utilizing yeast protein arrays.

AFFILIATION: Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas 66047, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20 RR016475

ACRONYM: RR

MEDLINETA: Mol Cell Proteomics

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