A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis.

A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis. Abstract Text:

T Fettah Kosar,Anna Tourovskaia,Xavier Figueroa-Masot,Marvin E Adams,Albert Folch,

The release of synaptogenic factors by the nerve terminal plays a central role in the aggregation of neurotransmitter receptors at the postsynaptic membrane, a precisely timed and localized process that is essential for the correct formation and functioning of the synapse. This process has been difficult to re-capitulate in cell culture because present cell stimulation methods do not have sufficient spatiotemporal control of the delivery of soluble signals. We cultured myotubes atop nanofabricated planar apertures (2-8 microm diameter) to focally stimulate the muscle cell membrane with neural agrin, a synaptogenic factor released by motor neurons during development. Focal agrin delivery through the apertures after myotube fusion results in local aggregation of acetylcholine receptors (AChRs) in the vicinity of the apertures, a process reminiscent of AChR clustering at innervation sites. Since the apertures are spatially organized in microarrays, multiple experiments can be run in parallel on one device. The technique has wide applicability in cell-cell communication studies and cell-based bioassays.

A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis. Publishing Authors By Initials

TF Kosar,A Tourovskaia,X Figueroa-Masot,ME Adams,A Folch,

For similar nervous system: synapses research abstracts see: nervous system: synapses research

PUBMED ID PMID:

MEDLINE DATE:

A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Lab on a chip

VOLUME: 6

Page Numbers: 632-8

Journal Abbreviation:

ISSN: 1473-0197

DAY: 22

MONTH: 03

YEAR: 2006

A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101128948

A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis. Keywords Mesh Terms:

KEYWORDS: Synapses

MESH TERMS: metabolism

Chemical & Substance for Abstract: A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis. Information

Substance Name: Receptors, Cholinergic

Registry Number: 0

Grant and Affiliation Information for A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis.

AFFILIATION: Department of Bioengineering, University of Washington, Campus Box 352255, Seattle, WA 98195-2255, USA.

Country: England

AGENCY: United States NCRR

GRANT: RR16302

ACRONYM: RR

MEDLINETA: Lab Chip

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis Related Publications

• A high-performance elastomeric patch clamp chip.
• A nanofabricated planar aperture as a mimic of the nerve-muscle contact during synaptogenesis.
• Biomolecular gradients in cell culture systems.
• Brain diphosphoninositide, a new phosphatide having inositol metadiphosphate as a constituent.
• Characterization and physical mapping of the porcine CDS1 and CDS2 genes.
• Characterization of genetic polymorphism in the goat beta-lactoglobulin gene.
• Comparison of protein components of different species and strains of Brucella.
• Complete fractionation of brain cephalin; isolation from it of phosphatidyl serine, phosphatidyl ethanolamine, and diphosphoinositide.
• Large-scale, multibreed, multitrait analyses of quantitative trait loci experiments: the case of porcine X chromosome.
• Localized acetylcholine receptor clustering dynamics in response to microfluidic focal stimulation with agrin.
• Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation.
• Measurement of cell migration in response to an evolving radial chemokine gradient triggered by a microvalve.
• The antiproliferative activity of melatonin in B65 rat dopaminergic neuroblastoma cells is related to the downregulation of cell cycle-related genes.
• The chemical structure of phospatidyl serine.
• Thudichum's Successors.
• [Not Available.]