A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase.

A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Abstract Text:

Jay P Stasser,Gnana S Siluvai,Amanda N Barry,Ninian J Blackburn,

Copper binding and X-ray aborption spectroscopy studies are reported on untagged human CCS (hCCS; CCS = copper chaperone for superoxide dismutase) isolated using an intein self-cleaving vector and on single and double Cys to Ala mutants of the hCCS MTCQSC and CSC motifs of domains 1 (D1) and 3 (D3), respectively. The results on the wild-type protein confirmed earlier findings on the CCS-MBP (maltose binding protein) constructs, namely, that Cu(I) coordinates to the CXC motif, forming a cluster at the interface of two D3 polypeptides. In contrast to the single Cys to Ser mutations of the CCS-MBP protein (Stasser, J. P., Eisses, J. F., Barry, A. N., Kaplan, J. H., and Blackburn, N. J. (2005) Biochemistry 44, 3143-3152), single Cys to Ala mutations in D3 were sufficient to eliminate cluster formation and significantly reduce CCS activity. Analysis of the intensity of the Cu-Cu cluster interaction in C244A, C246A, and C244/246A variants suggested that the nuclearity of the cluster was greater than 2 and was most consistent with a Cu4S6 adamantane-type species. The relationship among cluster formation, oligomerization, and metal loading was evaluated. The results support a model in which Cu(I) binding converts the apo dimer with a D2-D2 interface to a new dimer connected by cluster formation at two D3 CSC motifs. The predominance of dimer over tetramer in the cluster-containing species strongly suggests that the D2 dimer interface remains open and available for sequestering an SOD1 monomer. This work implicates the copper cluster in the reactive form and adds detail to the cluster nuclearity and how copper loading affects the oligomerization states and reactivity of CCS for its partner SOD1.

A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Publishing Authors By Initials

JP Stasser,GS Siluvai,AN Barry,NJ Blackburn,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biochemistry

VOLUME: 46

Page Numbers: 11845-56

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 29

MONTH: 09

YEAR: 2007

A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370623

A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase.

AFFILIATION: Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Sciences University, Beaverton, OR 97006-8291, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: P01 GM067166

ACRONYM: GM

MEDLINETA: Biochemistry

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A multinuclear copperI cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase Related Publications

• A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase.
• A newly recognised strain of Staphylococcus aureus associated with epidemics in six hospitals.
• A preschool program at East York Health Unit.
• A short history of dentistry in ancient times.
• A3 adenosine receptor signaling contributes to airway mucin secretion after allergen challenge.
• Breast-feeding Internet Resources.
• Cardiovascular survey methods.
• Coarctation of the aorta in a man, aged 63 years.
• Daidzein-rich isoflavone aglycones are potentially effective in reducing hot flashes in menopausal women.
• Diet and breast cancer recurrence.
• Disequilibrium due to a vitamin B6 megadose supplement.
• Expression and suppression of human telomerase RNA.
• FLUOROMETHOLONE (NSC-33001) AS A PALLIATIVE IN ADVANCED GASTROINTESTINAL CARCINOMA.
• Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice.
• Human cancer cells harbor T-stumps, a distinct class of extremely short telomeres.
• Insidious onset neck pain - a symptom not to be dismissed.
• Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor.
• Ligand dependent release of activated neutrophil collagenase--a role for surface bound IgG in the degradation of articular collagens.
• MONOSEMICARBAZONE OF ADRENOCHROME (ADRENOXYL) AND CATARACT SURGERY EFFECT ON CAPILLARY RESISTANCE AND INCIDENCE OF HYPHAEMA.
• One-solvent polymorph screen of carbamazepine.
• Proteins synthesized in medial hypothalamus and transported to midbrain in estrogen-treated female rats.
• Selected internet resources relating to trauma and violence in women and infants.
• Spatial turnover in the global avifauna.
• The Telotype Defines the Telomere State in Saccharomyces cerevisiae and Is Inherited as a Dominant Non-Mendelian Characteristic in Cells Lacking Telomerase.
• The low-fat imperative.
• The role of lead in the reduction of the erythrocyte sedimentation rate by hyaluronidase.
• Thymus organogenesis and development of the thymic stroma.
• Use of 2alpha-methyldihydrotestosterone propionate in treatment of advanced cancer of the breast: a group report.
• Using aliens to explore how our planet works.
• VINCRISTINE (NSC-67574) THERAPY OF ADULT PATIENTS WITH SOLID TUMORS.