A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG.

A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG. Abstract Text:

T Kohno,S Hashida,E Ishikawa,

An enzyme immunoassay of anti-insulin IgG in guinea pig serum was improved in sensitivity by reducing the non-specific binding of normal guinea pig IgG and enhancing the specific binding of anti-insulin IgG. Silicone rubber pieces or polystyrene balls were coated with normal rabbit IgG, followed by coupling of insulin using glutaraldehyde. The insulin-normal rabbit IgG-coated silicone rubber pieces or polystyrene balls were incubated with normal rabbit IgG and then with diluted guinea pig anti-insulin serum in the presence of normal rabbit IgG at a lower temperature (20 degrees C). Finally, the solid phases were incubated with rabbit (anti-guinea pig IgG) Fab'-horseradish peroxidase conjugate to measure the amount of guinea pig IgG bound. The detection limit of anti-insulin IgG in guinea pig serum was improved 10 to 100-fold compared to that of enzyme immunoassay performed by incubating insulin-bovine serum albumin-coated solid phases with diluted guinea pig anti-insulin serum at 37 degrees C and then with rabbit (anti-guinea pig IgG) Fab' conjugated to beta-D-galactosidase from Escherichia coli, according to a previous report (Kato, K., et al. (1978) J. Biochem. 84, 93-102).

A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG. Publishing Authors By Initials

T Kohno,S Hashida,E Ishikawa,

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

PUBMED ID PMID:

MEDLINE DATE:

A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 98

Page Numbers: 379-84

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1985

A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: immunology

Chemical & Substance for Abstract: A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG. Information

Substance Name: Serum Albumin, Bovine

Registry Number: 0

Grant and Affiliation Information for A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A more sensitive enzyme immunoassay of anti-insulin IgG in guinea pig serum with less non-specific binding of normal guinea pig IgG Related Publications

• Antegrade radical retropubic prostatectomy with preliminary ligation of vascular pedicles in 614 consecutive patients.
• Bone regeneration by recombinant human bone morphogenetic protein-2 in rat mandibular defects. An experimental model of defect filling.
• Cellular senescence and chromatin structure.
• Complete removal of a bronchial granular cell tumor by bronchoplasty.
• Development and validation of the finite element model for the human lower limb of pedestrians.
• Disruption of type 5 adenylyl cyclase enhances desensitization of cyclic adenosine monophosphate signal and increases Akt signal with chronic catecholamine stress.
• Ectopic bone formation by biphasic calcium phosphate (BCP) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2).
• Elevated IgG titers to periodontal pathogens related to Buerger disease.
• Er:YAG laser therapy for peri-implant infection: a histological study.
• Fabrication of Silicon Crystals for a Pin-Post Water-Cooling System at SPring-8.
• Fatal polymyositis associated with sarcoidosis.
• Focusing and reflection by a bent crystal for high-energy synchrotron radiation.
• Mathematical modeling of severe acute respiratory syndrome nosocomial transmission in Japan: the dynamics of incident cases and prevalent cases.
• Multiple morphea coexistent with atrophoderma of Pasini-Pierini (APP): APP could be abortive morphea.
• Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs.
• Position of exit X-rays from rotated-inclined double-crystal monochromators.
• Reference table predicting the outcome of subarachnoid hemorrhage in the elderly, stratified by age.
• Relationship between rapid cold-hardening and cold acclimation in the eggs of the yellow-spotted longicorn beetle, Psacothea hilaris.
• SPring-8 beamline control system.
• Should arterial infusion therapy be recommended positively for advanced hepatocellular carcinoma with portal vein tumor thrombosis?
• Stabilities and conformational transitions of various proteases in the presence of an organic solvent.
• Standard Transport Channels of X-ray Beamlines at SPring-8.
• Sweat duct carcinoma of lip with multiple cervical lymph nodes metastasis.
• Telomeric DNA length in cerebral gray and white matter is associated with longevity in individuals aged 70 years or older.
• The roles of copper transporters in cisplatin resistance.
• Upregulation of a desaturase is associated with the enhancement of cold hardiness in the onion maggot, Delia antiqua.
• [Comparison of atypical orofacial pain and temporomandibular disorders synovitis pain processing in the human brain using functional magnetic resonance imaging]
• [Ectopic bone induction in recombinant human bone morphogenetic protein-2 (rhBMP-2) combined with biphasic calcium phosphate (BCP)]
• [Production of interleukin-11 and interleukin-6 in cultured human gingival fibroblasts with the stimulation of lipopolysacchrides]
• [The role of ABC transporters in porphyrin biosynthesis and heme metabolism]