A monoclonal antibody (1D12) defines novel distribution patterns of prion protein (PrP) as granules in nucleus.

A monoclonal antibody (1D12) defines novel distribution patterns of prion protein (PrP) as granules in nucleus. Research Abstract Details 

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A monoclonal antibody (1D12) defines novel distribution patterns of prion protein (PrP) as granules in nucleus. Abstract Text:

Tomoko Hosokawa,Kotaro Tsuchiya,Ichiro Sato,Natsumi Takeyama,Susumu Ueda,Yuichi Tagawa,Kumiko M Kimura,Izuru Nakamura,Guoying Wu,Akikazu Sakudo,Cristina Casalone,Maria Mazza,Maria Caramelli,Hidehiro Takahashi,Tetsutaro Sata,Katsuaki Sugiura,Andreina Baj,Antonio Toniolo,Takashi Onodera,Tomoko Hosokawa,Kotaro Tsuchiya,Ichiro Sato,Natsumi Takeyama,Susumu Ueda,Yuichi Tagawa,Kumiko M Kimura,Izuru Nakamura,Guoying Wu,Akikazu Sakudo,Cristina Casalone,Maria Mazza,Maria Caramelli,Hidehiro Takahashi,Tetsutaro Sata,Katsuaki Sugiura,Andreina Baj,Antonio Toniolo,Takashi Onodera,

A monoclonal antibody (mAb) panel to bovine prion protein (PrP) was studied by immunoblotting and immunohistochemistry for scrapie and bovine spongiform encephalopathy. A mAb panel recognized both normal (PrP(C)) and abnormal (PrP(Sc)) isoforms of PrP in murine, ovine and bovine brain tissues. Interestingly, an anti-bovine PrP mAb, 1D12, prepared by immunizing PrP gene-knockout mice with a synthetic polypeptides corresponding to codons 153-166 of the bovine PrP gene showed novel patterns of reactivity for prion-uninfected neuronal cells. An enzyme-linked immunosorbent assay-mapping of the mAb epitopes resulted in a reaction of monoclonal 1D12 to YEDRY and M corresponding to amino acids 156-160 and 165 of bovine PrP. Several patterns of bovine PrP(C) distribution in PrP-deficient neuronal cells (HpL3-4) transfected with bovine PrP were observed after different fixation methods. Stained cell surface was observed after formalin fixation by immunofluorescent assay of 1D12 with confocal microscopy, whereas granules in nucleus were stained after acetone fixation. No reactivity in the nucleus was observed to HpL3-4, or HpL3-4mPrP cells expressing mouse PrP. This is the first paper that has reported the detection of the PrP(C) at both cell surface and nuclei of prion-uninfected cell line.

A monoclonal antibody (1D12) defines novel distribution patterns of prion protein (PrP) as granules in nucleus. Publishing Authors By Initials

T Hosokawa,K Tsuchiya,I Sato,N Takeyama,S Ueda,Y Tagawa,KM Kimura,I Nakamura,G Wu,A Sakudo,C Casalone,M Mazza,M Caramelli,H Takahashi,T Sata,K Sugiura,A Baj,A Toniolo,T Onodera,T Hosokawa,K Tsuchiya,I Sato,N Takeyama,S Ueda,Y Tagawa,KM Kimura,I Nakamura,G Wu,A Sakudo,C Casalone,M Mazza,M Caramelli,H Takahashi,T Sata,K Sugiura,A Baj,A Toniolo,T Onodera,

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A monoclonal antibody (1D12) defines novel distribution patterns of prion protein (PrP) as granules in nucleus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 366

Page Numbers: 657-63

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 7

MONTH: 12

YEAR: 2007

A monoclonal antibody (1D12) defines novel distribution patterns of prion protein (PrP) as granules in nucleus. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for A monoclonal antibody (1D12) defines novel distribution patterns of prion protein (PrP) as granules in nucleus.

AFFILIATION: Nippon Institute for Biological Science, Ome 198-0024, Tokyo, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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