A molecular chaperone complex at the lysosomal membrane is required for protein translocation.

A molecular chaperone complex at the lysosomal membrane is required for protein translocation. Research Abstract Details 

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A molecular chaperone complex at the lysosomal membrane is required for protein translocation. Abstract Text:

A group of cytosolic proteins are targeted to lysosomes for degradation in response to serum withdrawal or prolonged starvation by a process termed chaperone-mediated autophagy. In this proteolytic pathway little is known about how proteins are translocated across lysosomal membranes. We now show that an isoform of the constitutively expressed protein of the heat shock family of 70 kDa (Hsc70) is associated with the cytosolic side of the lysosomal membrane where it binds to substrates of this proteolytic pathway. Results from coimmunoprecipitation and colocalization studies indicate that this molecular chaperone forms complexes with other molecular chaperones and cochaperones, including Hsp90, Hsp40, the Hsp70-Hsp90 organizing protein (Hop), the Hsp70-interacting protein (Hip), and the Bcl2-associated athanogene 1 protein (BAG-1). Antibodies against Hip, Hop, Hsp40 and Hsc70 block transport of protein substrates into purified lysosomes.

A molecular chaperone complex at the lysosomal membrane is required for protein translocation. Publishing Authors By Initials

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A molecular chaperone complex at the lysosomal membrane is required for protein translocation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of cell science

VOLUME: 114

Page Numbers: 2491-9

Journal Abbreviation: J. Cell. Sci.

ISSN: 0021-9533

DAY: 11

MONTH: Jul

YEAR: 2001

A molecular chaperone complex at the lysosomal membrane is required for protein translocation. Information

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LANGUAGE: eng

NlmUniqueID: 52457

A molecular chaperone complex at the lysosomal membrane is required for protein translocation. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: A molecular chaperone complex at the lysosomal membrane is required for protein translocation. Information

Substance Name: hopscotch protein, Drosophila

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for A molecular chaperone complex at the lysosomal membrane is required for protein translocation.

AFFILIATION: The Sackler School of Graduate Biomedical Sciences, Department of Cellular and Molecular Physiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.

Country: England

AGENCY: United States NIA

GRANT: AG06116

ACRONYM: AG

MEDLINETA: J Cell Sci

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