A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane.

A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. Research Abstract Details 

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A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. Abstract Text:

Yeong-Ray Wen,Geng-Chang Yeh,Bai-Chuang Shyu,Qing-Dong Ling,Kuo-Ching Wang,Ta-Liang Chen,Wei-Zen Sun,

The use of anesthetics in acupuncture analgesia is controversial. We evaluate a steady-state light anesthesia model to test whether minimal stress manipulation and reliable measurement of analgesia could be simultaneously achieved during electroacupuncture (EA) in animals. A series of experiments were performed. Firstly, EA compliance and tail-flick latencies (TFL) were compared in rats under 0.1%, 0.3%, 0.5%, 0.7%, or 1.1% halothane for 120min. Under 0.5% halothane, TFL were then measured in groups receiving EA at intensity of 3, 10 or 20 volt (V), 1 or 2mg/kg morphine, 20V EA plus naloxone, or control. Subsequently, the effect of EA on formalin-induced hyperalgesia was tested and c-fos expression in the spinal dorsal horn was analyzed. Rats exhibited profound irritable behaviors and highly variable TFL under 0.1% or 0.3% halothane, as well as a time-dependent increase of TFL under 0.7% or 1.1% halothane. TFL remained constant at 0.5% halothane, and needle insertion and electrical stimulation were well tolerated. Under 0.5% halothane, EA increased TFL and suppressed formalin-induced hyperalgesia in an intensity-dependent and naloxone-reversible manner. EA of 20V prolonged TFL by 74%, suppressed formalin-induced hyperalgesia by 32.6% and decreased c-fos expression by 29.7% at the superficial and deep dorsal horn with statistically significant difference. In conclusion, 0.5% halothane provides a steady-state anesthetic level which enables the humane application of EA stimulus with the least interference on analgesic assessment. This condition serves as a minimal stress EA model in animals devoid of stress-induced analgesia while maintaining physiological and biochemical response in the experiment.

A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. Publishing Authors By Initials

YR Wen,GC Yeh,BC Shyu,QD Ling,KC Wang,TL Chen,WZ Sun,

For similar animal structures: tail research abstracts see: animal structures: tail research

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A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: European journal of pain (London, England)

VOLUME: 11

Page Numbers: 733-42

Journal Abbreviation:

ISSN: 1090-3801

DAY: 10

MONTH: 01

YEAR: 2007

A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. Information

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LANGUAGE: eng

NlmUniqueID: 9801774

A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. Keywords Mesh Terms:

KEYWORDS: Tail

MESH TERMS: prevention & control

Chemical & Substance for Abstract: A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. Information

Substance Name: Halothane

Registry Number: 151-67-7

Grant and Affiliation Information for A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane.

AFFILIATION: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.

Country: England

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MEDLINETA: Eur J Pain

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