A simple and low-cost method to obtain cyanobacterial toxin microcystin-LR (MC-LR) was developed. A new strain of Microcystis aeruginosa, named DC-1, producing microcystin-LR but not microcystin-RR, was separated from the field blooming algae samples of Dianchi Lake, in southwest of China. Following three times' freeze and thaw treatment, the cultivated DC-1 cells were extracted with 40% methanol in water. The extract was centrifuged and the supernatant applied to a Hydrophilic-Lipophilic Balance (HLB) SPE cartridge. Eluted impurities with a certain gradient from 30% to 50% methanol in water, MC-LR was finally eluted from the HLB cartridge with 60% methanol in water, and samples containing 3.85% to 14.8% of MC-LR were obtained. These MC-LR samples may be used in adsorption and biodegradation experiments instead of using expensive standard reagents.
A method to extract algae toxin of microcystin-LR. Publishing Authors By Initials
A method to extract algae toxin of microcystin-LR. Journal Published:
PUBLICATION TYPE: Research Support, Non-U.S. Gov
Journal: Journal of environmental sciences (China)
VOLUME: 16
Page Numbers: 694-6
Journal Abbreviation: J Environ Sci (China)
ISSN: 1001-0742
DAY: 8
MONTH: 02
YEAR: 2004
A method to extract algae toxin of microcystin-LR. Information
Number of References:
LANGUAGE: eng
NlmUniqueID: 100967627
A method to extract algae toxin of microcystin-LR. Keywords Mesh Terms:
KEYWORDS: Phosphoprotein Phosphatases
MESH TERMS: antagonists & inhibitors
Chemical & Substance for Abstract: A method to extract algae toxin of microcystin-LR. Information
Substance Name: Phosphoprotein Phosphatases
Registry Number: EC 3.1.3.16
Grant and Affiliation Information for A method to extract algae toxin of microcystin-LR.
AFFILIATION: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Country: Netherlands
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MEDLINETA: J Environ Sci (China)
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