A method for the generation of glycoprotein mimetics.

A method for the generation of glycoprotein mimetics. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A method for the generation of glycoprotein mimetics. Abstract Text:

Haitian Liu,Lei Wang,Ansgar Brock,Chi-Huey Wong,Peter G Schultz,

A general method for preparing glycoprotein mimetics with defined glycan structure using the Z domain protein as an example is reported. An unnatural amino acid containing the keto group was site-specifically incorporated into a target protein, Z domain, in response to the amber nonsense codon with high translational fidelity and efficiency. An aminooxy saccharide derivative was then selectively coupled to this genetically encoded keto group. The resulting saccharide core was elaborated to a glycan complex with glycosyltransferases. Alternatively, aminooxy analogues of more complicated glycans were prepared and directly attached to the keto group. Homogeneous glycoprotein mimetics thus prepared should prove useful for the study of carbohydrate effects on glycoprotein structure and function. This method may also lead to the production of glycoprotein therapeutics from Escherichia coli.

A method for the generation of glycoprotein mimetics. Publishing Authors By Initials

H Liu,L Wang,A Brock,CH Wong,PG Schultz,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleotides: uracil nucleotides: uridine diphosphate: uridine diphosphate sugars: uridine diphosphate galactose research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleotides: uracil nucleotides: uridine diphosphate: uridine diphosphate sugars: uridine diphosphate galactose research

PUBMED ID PMID:

MEDLINE DATE:

A method for the generation of glycoprotein mimetics. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 1702-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 19

MONTH: Feb

YEAR: 2003

A method for the generation of glycoprotein mimetics. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

A method for the generation of glycoprotein mimetics. Keywords Mesh Terms:

KEYWORDS: Uridine Diphosphate Galactose

MESH TERMS: chemistry

Chemical & Substance for Abstract: A method for the generation of glycoprotein mimetics. Information

Substance Name: N-Acetyllactosamine Synthase

Registry Number: EC 2.4.1.90

Grant and Affiliation Information for A method for the generation of glycoprotein mimetics.

AFFILIATION: Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM62159

ACRONYM: GM

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A method for the generation of glycoprotein mimetics Related Publications

• A genetically encoded photocaged tyrosine.
• A phosphoproteomic analysis of the ErbB2 receptor tyrosine kinase signaling pathways.
• Altered Toll-like receptor 9 responses in circulating B cells at the onset of extensive chronic graft-versus-host disease.
• Bovine parthenogenetic blastocysts following in vitro maturation and oocyte activation with ethanol.
• Ca2+ oscillatory pattern in fertilized mouse eggs affects gene expression and development to term.
• Defending the psychiatric malpractice suicide.
• Dose-response relationships in gene expression profiles in rainbow trout, Oncorhyncus mykiss, exposed to ethynylestradiol.
• Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern.
• From humble beginnings...
• Gene expression patterns in rainbow trout, Oncorhynchus mykiss, exposed to a suite of model toxicants.
• Genetic incorporation of unnatural amino acids into proteins in mammalian cells.
• Global cerebral blood flow in relation to cognitive performance and reserve in subjects with mild memory deficits.
• Glucocorticoids stimulate ENaC upregulation in bovine mammary epithelium.
• History of the international embryo transfer society Part II.
• Implication of nucleolar protein SURF6 in ribosome biogenesis and preimplantation mouse development.
• Incorporation of a follicle stimulating hormone used for embryo transfer in cattle into multilamellar liposomes.
• Modeling the brain-pituitary-gonad axis in salmon.
• Neurodevelopmental and behavioral issues in Williams syndrome.
• Paleohistopathology of bone: A new approach to the study of ancient diseases.
• Recombinant expression of selectively sulfated proteins in Escherichia coli.
• Structural factors influencing linear M-H-M bonding in bis(dialkylphosphino)methane complexes of nickel.
• Synthesis, structure, and properties of low-spin manganese(III)-poly(pyrazolyl)borate complexes.
• Temporal changes in gene expression in rainbow trout exposed to ethynyl estradiol.
• The KAP1 corepressor functions to coordinate the assembly of de novo HP1-demarcated microenvironments of heterochromatin required for KRAB zinc finger protein-mediated transcriptional repression.
• The cardioprotective effect of the low molecular weight isoform of fibroblast growth factor-2: the role of JNK signaling.
• The day of the week when you were born in 700 ms: calendar computation in an Autistic savant.
• The effects of antibodies on stilbene excited-state energetics.
• Thyroid hormone regulates endogenous amyloid-beta precursor protein gene expression and processing in both in vitro and in vivo models.
• Tobacco or health: A global status report.
• Transforming growth factor-beta enhances connective tissue repair in perforated rat mesentery but not peritoneal macrophage chemotaxis.