A method for the covalent capture and screening of diverse small molecules in a microarray format.

A method for the covalent capture and screening of diverse small molecules in a microarray format. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A method for the covalent capture and screening of diverse small molecules in a microarray format. Abstract Text:

James E Bradner,Olivia M McPherson,Angela N Koehler,

This protocol describes a robust method for the covalent capture of small molecules with diverse reactive functional groups in microarray format, and outlines a procedure for probing small-molecule microarrays (SMMs) with proteins of interest. A vapor-catalyzed, isocyanate-mediated surface immobilization scheme is used to attach bioactive small molecules, natural products and small molecules derived from diversity-oriented synthesis pathways. Additionally, an optimized methodology for screening SMMs with purified proteins and cellular lysates is described. Finally, a suggested model for data analysis that is compatible with commercially available software is provided. These procedures enable a platform capability for discovering novel interactions with potential applications to immunoglobulin profiling, comparative analysis of cellular states and ligand discovery. With the appropriate materials and experimental setup, the printing of SMMs can be completed in 14 hours over 3 days. Screening and data analysis requires 2 days. A detailed timeline is provided.

A method for the covalent capture and screening of diverse small molecules in a microarray format. Publishing Authors By Initials

JE Bradner,OM McPherson,AN Koehler,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research

PUBMED ID PMID:

MEDLINE DATE:

A method for the covalent capture and screening of diverse small molecules in a microarray format. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature protocols

VOLUME: 1

Page Numbers: 2344-52

Journal Abbreviation: Nat Protoc

ISSN: 1750-2799

DAY: 24

MONTH: 03

YEAR: 2006

A method for the covalent capture and screening of diverse small molecules in a microarray format. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

A method for the covalent capture and screening of diverse small molecules in a microarray format. Keywords Mesh Terms:

KEYWORDS: Protein Binding

MESH TERMS: methods

Chemical & Substance for Abstract: A method for the covalent capture and screening of diverse small molecules in a microarray format. Information

Substance Name: Ligands

Registry Number: 0

Grant and Affiliation Information for A method for the covalent capture and screening of diverse small molecules in a microarray format.

AFFILIATION: Broad Institute of Harvard and MIT, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Nat Protoc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A method for the covalent capture and screening of diverse small molecules in a microarray format Related Publications

• A SacB mutagenesis strategy reveals that the Bartonella quintana variably expressed outer membrane proteins are required for bloodstream infection of the host.
• A new function in translocation for the mitochondrial i-AAA protease Yme1: import of polynucleotide phosphorylase into the intermembrane space.
• A pipeline for ligand discovery using small-molecule microarrays.
• Acid-base balance of the domestic Turkey during thermal panting.
• Bacteremia, fever, and splenomegaly caused by a newly recognized bartonella species.
• Combined effects of termiticides and mechanical stress on chlorinated polyvinyl chloride (CPVC) pipe.
• Detecting binding interactions using microarrays of natural product extracts.
• Dopamine receptor modulation of hypoxic-ischemic neuronal injury in striatum of newborn piglets.
• FOOD INSPECTION AND FOOD IN RELATION TO PUBLIC HEALTH.
• Fluorometric measurement of aflatoxins.
• In vitro analysis of yeast mitochondrial protein import.
• Influence of duration of focal cerebral ischemia and neuronal nitric oxide synthase on translocation of apoptosis-inducing factor to the nucleus.
• Interaction of mechanisms involving epoxyeicosatrienoic acids, adenosine receptors, and metabotropic glutamate receptors in neurovascular coupling in rat whisker barrel cortex.
• Lumbosacral plexopathy associated with aortoiliac occlusive disease.
• Milwaukee's Well Advertised Gastrointestinal Epidemic.
• Modern Trends in Public Health Administration in Cities.
• Natural orifice transluminal endoscopic surgery (NOTES) in the mediastinum: long-term survival animal experiments in transesophageal access, including minor surgical procedures.
• PRELIMINARY REPORT ON THE INFLUENZA EPIDEMIC IN CHICAGO.
• PUBLIC HEALTH ADMINISTRATION: ANNUAL REPORTS.
• Proteomic and immunoblot analyses of Bartonella quintana total membrane proteins identify antigens recognized by sera from infected patients.
• Recent Experiences in Scarlet Fever Control.
• Relationship of circulating total homocysteine and C-reactive protein to trabecular bone in postmenopausal women.
• The alternative sigma factor sigmaH is required for toxin gene expression by Bacillus anthracis.
• Tim54p connects inner membrane assembly and proteolytic pathways in the mitochondrion.
• [New method for the isolation of the poliomyelitis virus from the blood of humans affected by infantile paralysis.]
• [Not Available.]
• [Not Available.]
• [Paper chromato-oscillopolarographic determination of vanillin and ethylvanillin in food.]
• [The roll of leukocytes as virus carriers.]
• plcR papR-independent expression of anthrolysin O by Bacillus anthracis.