A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells.

A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells. Research Abstract Details 

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A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells. Abstract Text:

Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication.

A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells. Publishing Authors By Initials

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A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of microbiological methods

VOLUME: 72

Page Numbers: 321-5

Journal Abbreviation: J. Microbiol. Methods

ISSN: 0167-7012

DAY: 12

MONTH: 01

YEAR: 2008

A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells. Information

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LANGUAGE: eng

NlmUniqueID: 8306883

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Grant and Affiliation Information for A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells.

AFFILIATION: Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, United States.

Country: Netherlands

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MEDLINETA: J Microbiol Methods

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