A method for micrometer resolution patterning of primary culture neurons for SPM analysis.

A method for micrometer resolution patterning of primary culture neurons for SPM analysis. Research Abstract Details 

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A method for micrometer resolution patterning of primary culture neurons for SPM analysis. Abstract Text:

P Degenaar,B L Pioufle,L Griscom,A Tixier,Y Akagi,Y Morita,Y Murakami,K Yokoyama,H Fujita,E Tamiya,

In this work we present a method for ultra-fine patterning of primary culture neuron cell growth, which is compatible for scanning near-field optical atomic force microscopy (SNOAM) analysis. SNOAM uses near-field optics to break the fundamental diffraction limit imposed on normal microscopy. SNOAM can achieve sub-100 nm optical resolutions, but requires transparent, open substrates. The ability to do physiological measurements on patterns of neurons, combined with ultra high resolution optical and fluorescent analysis, is useful in the study of long-term potentiation. The patterning method consists of chemical guidance with an element of physical confinement and allows for ultra-fine patterning of neural growth on transparent glass substrates. Substrates consist of microfabricated perfluoropolymer barrier structures on glass. Poly-L-lysine was selectively deposited using a silicone-based microfluidic stencil aligned to the perfluoropolymer/glass substrate. Primary culture neurons were extracted from 8-day-old chicks and grown for 3 days to form good networks. This patterning system shows very specific growth with patterning separations down to the level of individual neurites. Fluorescent imaging was carried out on both cell viability during growth and immuno-tagged microtubule-associated proteins on the neurites. Neurons inside the patterned structures were imaged and analyzed with a tapping mode SNOAM.

A method for micrometer resolution patterning of primary culture neurons for SPM analysis. Publishing Authors By Initials

P Degenaar,BL Pioufle,L Griscom,A Tixier,Y Akagi,Y Morita,Y Murakami,K Yokoyama,H Fujita,E Tamiya,

For similar proteins: membrane proteins: receptors, cell surface: receptors, neurotransmitter: receptors, amino acid: receptors, glutamate: receptors, n-methyl-d-aspartate research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, neurotransmitter: receptors, amino acid: receptors, glutamate: receptors, n-methyl-d-aspartate research

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A method for micrometer resolution patterning of primary culture neurons for SPM analysis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 130

Page Numbers: 367-76

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 2001

A method for micrometer resolution patterning of primary culture neurons for SPM analysis. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A method for micrometer resolution patterning of primary culture neurons for SPM analysis. Keywords Mesh Terms:

KEYWORDS: Receptors, N-Methyl-D-Aspartate

MESH TERMS: analysis

Chemical & Substance for Abstract: A method for micrometer resolution patterning of primary culture neurons for SPM analysis. Information

Substance Name: Receptors, N-Methyl-D-Aspartate

Registry Number: 0

Grant and Affiliation Information for A method for micrometer resolution patterning of primary culture neurons for SPM analysis.

AFFILIATION: The School of Materials Science, Japan Advanced Institute of Science and Technology, Hokuriku, Asahidai, Tatsunokuchi, Ishikawa 923-1292, Japan. tamiya@jaist.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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