A method for incorporating labeled lacithin into serum low density lipoproteins in vitro.

A method for incorporating labeled lacithin into serum low density lipoproteins in vitro. Research Abstract Details 

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A method for incorporating labeled lacithin into serum low density lipoproteins in vitro. Abstract Text:

S Yokoyama,M Takayama,Y Akanuma,

A sonicated dispersion of [14C]lecithin was incubated with high density lipoproteins (HDL) coupled to Sepharose. After washing the gels thoroughly with a buffer, the gels were incubated with low density lipoproteins (LDL); [14C]lecithin was transferred from the sonicated dispersion via HDL-Sepharose to the LDL. The LDL fraction thus prepared showed no contamination with lecithin dispersion or HDL. The lecithin:cholesterol acyltransferase (LCAT) reaction could be completely inhibited during preparation, and the net recovery of radioactivity in LDL was 16% of that of the original lecithin dispersion. The [14C]lecithin in the washed HDL-Sepharose was shown to be a substrate of the LCAT reaction in vitro.

A method for incorporating labeled lacithin into serum low density lipoproteins in vitro. Publishing Authors By Initials

S Yokoyama,M Takayama,Y Akanuma,

For similar phosphatidic acids: glycerophospholipids: phosphatidylcholines research abstracts see: phosphatidic acids: glycerophospholipids: phosphatidylcholines research

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A method for incorporating labeled lacithin into serum low density lipoproteins in vitro. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 1227-30

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1977

A method for incorporating labeled lacithin into serum low density lipoproteins in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A method for incorporating labeled lacithin into serum low density lipoproteins in vitro. Keywords Mesh Terms:

KEYWORDS: Phosphatidylcholines

MESH TERMS: blood

Chemical & Substance for Abstract: A method for incorporating labeled lacithin into serum low density lipoproteins in vitro. Information

Substance Name: Phosphatidylcholine-Sterol O-Acyltransfe

Registry Number: EC 2.3.1.43

Grant and Affiliation Information for A method for incorporating labeled lacithin into serum low density lipoproteins in vitro.

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Country: JAPAN

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MEDLINETA: J Biochem

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