A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts.

A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts. Research Abstract Details 

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A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts. Abstract Text:

R M Strojek,M A Reed,J L Hoover,T E Wagner,

Variable conditions were tested to determine an in-vitro cultivation method for the formation of morphologically undifferentiated embryonic stem cells from the inner cell mass (ICM) derived outgrowth of porcine blastocysts. Although all 16 Day-9 embryos failed to form colonies, 14 such colonies were obtained from a total of 69 Day-10 embryos when they were co-cultivated with porcine uterine fibroblast (PUF) cells over a 6-day period. The best results were obtained in Dulbecco's modified Eagle medium (DMEM) with 10% fetal calf serum and 10% porcine serum supplemented with bovine insulin and beta-mercaptoethanol, in which six out of seven embryos formed adequate ICM-derived colonies. Since murine fibroblasts were not found to be suitable feeder cells in this procedure, an endocrine synergistic interaction, which promotes embryonic attachment and colony formation, between porcine blastocysts and PUF cells is hypothesized. Continued propagation of the ICM-derived cells was not dependent on these factors; a total of seven cell lines were obtained after three to five subsequent passages on murine feeder-layers that resembled morphologically undifferentiated embryonic cells.

A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts. Publishing Authors By Initials

RM Strojek,MA Reed,JL Hoover,TE Wagner,

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A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 33

Page Numbers: 901-13

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Apr

YEAR: 1990

A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts. Information

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LANGUAGE: eng

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Grant and Affiliation Information for A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts.

AFFILIATION: Edison Animal Biotechnology Center Ohio University, Wilson Hall - West Green Athens, OH 45701 USA.

Country: United States

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MEDLINETA: Theriogenology

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