A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study.

A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study. Research Abstract Details 

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A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study. Abstract Text:

A standard method for the detection of Enterobacteriaceae was modified for the presumptive detection of Enterobacter sakazakii, and the modified method was validated in an interlaboratory trial with 16 laboratories from 8 European countries. The modification included a differential-elective medium for the isolation of E. sakazakii, consisting of nutrient agar (NA) supplemented with 4-methyl-umbelliferyl alpha-D-glucoside (alpha-MUG). A 25 g sample was added to 225 mL buffered peptone water. After incubation at 35 degrees or 37 degrees C for 16 or 20 h, 10 mL nonselective enrichment was transferred into 90 mL selective enrichment. The selective enrichment was streaked on violet-red bile glucose agar (VRBGA) and incubated at 37 degrees C for 24 h. It was streaked in parallel on NA plates supplemented with alpha-MUG at 50 mg/L and incubated at 25 degrees C for 16 h, and afterwards for an additional 24 h at room temperature in the dark. E. sakazakii appeared as vivid yellow colonies under normal light and showed blue/violet fluorescence under UV light on NA + alpha-MUG plates. Validation samples represented powdered infant formula without E. sakazakii (blanks) and with low (1-10 colony-forming units [CFU]/25 g) and medium (1-10 CFU/g) contamination levels. All samples contained Pseudomonas aeruginosa and Lactobacillus spp. as background flora. The specificity for blank samples was 100%. The sensitivity of the low contamination level was similar for VRBGA and NA + alpha-MUG, i.e., 66.7% (66.7% accordance, 53.9% concordance). For the medium level the sensitivities were 96.7% (93.3% accordance, 93.5% concordance) for VRBGA and 98.3% (96.9% accordance, 96.9% concordance) for NA + alpha-MUG.

A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study. Publishing Authors By Initials

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

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A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 87

Page Numbers: 604-13

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study. Information

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LANGUAGE: eng

NlmUniqueID: 9215446

A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: chemistry

Chemical & Substance for Abstract: A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study. Information

Substance Name: Hymecromone

Registry Number: 90-33-5

Grant and Affiliation Information for A medium for the presumptive detection of Enterobacter sakazakii in infant formula: interlaboratory study.

AFFILIATION: Central Science Laboratory, Department for Environment, Food and Rural Affairs, Sand Hutton, York YO41 1LZ, United Kingdom. renata.leuschner@cec.eu.int

Country: United States

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MEDLINETA: J AOAC Int

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