A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment.

A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment. Research Abstract Details 

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A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment. Abstract Text:

Richard T Pomerantz,Dmitry Temiakov,Michael Anikin,Dmitry G Vassylyev,William T McAllister,

Transcription errors by T7 RNA polymerase (RNAP) may occur as the result of a mechanism in which the template base two positions downstream of the 3' end of the RNA (the TSn+1 base) is utilized during two consecutive nucleotide-addition cycles. In the first cycle, misalignment of the template strand leads to incorporation of a nucleotide that is complementary to the TSn+1 base. In the second cycle, the template is realigned and the mismatched primer is efficiently extended, resulting in a substitution error. Proper organization of the transcription bubble is required for maintaining the correct register of the DNA template, as the presence of a complementary nontemplate strand opposite the TSn+1 base suppresses template misalignment. Our findings for T7 RNAP are in contrast to related DNA polymerases of the Pol I type, which fail to extend mismatches efficiently and generate predominantly deletion errors as a result of template-strand misalignment.

A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment. Publishing Authors By Initials

RT Pomerantz,D Temiakov,M Anikin,DG Vassylyev,WT McAllister,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular cell

VOLUME: 24

Page Numbers: 245-55

Journal Abbreviation: Mol. Cell

ISSN: 1097-2765

DAY: 20

MONTH: Oct

YEAR: 2006

A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9802571

A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment. Information

Substance Name: DNA-Directed DNA Polymerase

Registry Number: EC 2.7.7.7

Grant and Affiliation Information for A mechanism of nucleotide misincorporation during transcription due to template-strand misalignment.

AFFILIATION: Department of Microbiology and Immunology, The State University of New York Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM74252

ACRONYM: GM

MEDLINETA: Mol Cell

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