A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data.

A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data. Research Abstract Details 

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A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data. Abstract Text:

Hua Xu,Michael A Freitas,

BACKGROUND: Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has become one of the most used tools in mass spectrometry based proteomics. Various algorithms have since been developed to automate the process for modern high-throughput LC-MS/MS experiments. RESULTS: A probability based statistical scoring model for assessing peptide and protein matches in tandem MS database search was derived. The statistical scores in the model represent the probability that a peptide match is a random occurrence based on the number or the total abundance of matched product ions in the experimental spectrum. The model also calculates probability based scores to assess protein matches. Thus the protein scores in the model reflect the significance of protein matches and can be used to differentiate true from random protein matches. CONCLUSION: The model is sensitive to high mass accuracy and implicitly takes mass accuracy into account during scoring. High mass accuracy will not only reduce false positives, but also improves the scores of true positive matches. The algorithm is incorporated in an automated database search program MassMatrix.

A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data. Publishing Authors By Initials

H Xu,MA Freitas,

For similar investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research abstracts see: investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research

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A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: BMC bioinformatics

VOLUME: 8

Page Numbers: 133

Journal Abbreviation: BMC Bioinformatics

ISSN: 1471-2105

DAY: 20

MONTH: 04

YEAR: 2007

A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data. Information

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LANGUAGE: eng

NlmUniqueID: 100965194

A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data. Keywords Mesh Terms:

KEYWORDS: Tandem Mass Spectrometry

MESH TERMS: methods

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Grant and Affiliation Information for A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data.

AFFILIATION: Department of Chemistry, the Ohio State University, Columbus 43210, OH, USA. xu.171@osu.edu <xu.171@osu.edu>

Country: England

AGENCY: United States NCI

GRANT: CA107106

ACRONYM: CA

MEDLINETA: BMC Bioinformatics

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