A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk.

A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Abstract Text:

Bacteriological culture as a diagnostic tool for chronic infections with Staphylococcus aureus intramammary infection is not completely satisfactory. The cyclical shedding pattern of Staph. aureus with intervals of low excretion is considered to be the main reason. We recently developed a novel assay for Staph. aureus in milk, based on real-time quantitative PCR (QPCR). In a longitudinal study of chronically infected cows we evaluated the diagnostic properties of this test under field conditions. Diagnostic sensitivity of the novel test proved to be very high with a value of 99.4%; diagnostic specificity was 97.1%. In addition, the shedding patterns of Staph. aureus for the sampling period were analyzed. Using log(10)-transformed QPCR data and plotting them across sampling time revealed a sinusoidal shedding pattern in 6 of 11 naturally infected quarters. Shedding patterns obtained by QPCR and by bacteriological culture were synchronous. In conclusion, the novel test has a very high diagnostic sensitivity and specificity so that quarters chronically infected with Staph. aureus are reliably detected, independent from their actual shedding quantity.

A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of dairy science

VOLUME: 91

Page Numbers: 1893-902

Journal Abbreviation: J. Dairy Sci.

ISSN: 1525-3198

DAY: 18

MONTH: May

YEAR: 2008

A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985126

A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk.

AFFILIATION: Clinic for Ruminants, Department of Clinical Veterinary Medicine, Vetsuisse-Faculty of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Berne, Switzerland.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Dairy Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk Related Publications

• "Phytoscreening": the use of trees for discovering subsurface contamination by VOCs.
• A longitudinal field study to evaluate the diagnostic properties of a quantitative real-time polymerase chain reaction-based assay to detect Staphylococcus aureus in milk.
• ALKALINE DIURESIS FOR ASPIRIN POISONING.
• Albuterol vs. levalbuterol for asthma treatment in children.
• An appraisal of the developmental deformities in cleft-palate and cleft-lip individuals.
• An improved medium for pyocyanin production by Pseudomonas aeruginosa.
• Choosing one PPI treatment over another.
• Craniofacial morphology in cleft palate and cleft lip deformities.
• Cysticercus bovis parasitizing wild ruminants in the republic of Chad.
• EXTRACTION IN ORTHODONTICS.
• Endoparasites in domestic and wild animals of the Central African Republic (CAR).
• Experimental physiology of a heart-lung machine in parallel with normal circulation.
• Increased LF spectrum power density loudspeaker system.
• Isotope correction of mass spectrometry profiles.
• Orthodontic therapy an exercise in decision making.
• Physical therapists' perspectives on the role and effectiveness of continuing education.
• Reciprocal changes in blood flow of the limbs and rectum during general anaesthesia.
• Separation and determination of trace amounts of bacteriostats using a continuous distillation extraction apparatus.
• Skin and soft tissue infections caused by community-acquired methicillin-resistant Staphylococcus aureus.
• Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis.
• [Appendicitis symptoms as a sequel to enteral Pasteurella pseudotuberculosis infection.]
• [Carcinoma of the prostate: a review (author's transl)]
• [Do results of surgical treatment of cancer clinically localized to the prostate justify early screening?]
• [Not Available.]
• [Occult pathology of renal system duplication]
• [Parasitic helminths in various domestic and wild animals in Chad]
• [Role of nutritional factor in bovine and ovine distomatosis caused by Fasciola gigantica]
• [Staghorn lithiasis (author's transl)]
• [The curative treatment of prostatic carcinoma (author's transl)]
• [What does a chronic pyelonephritis scar mean?]