A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno.

A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. Research Abstract Details 

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A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. Abstract Text:

Mark J Snee,Dianne Harrison,Nan Yan,Paul M Macdonald,

In Drosophila, posterior embryonic body patterning and germ cell formation rely on Oskar, a protein that is concentrated at the posterior pole of the oocyte. A program of mRNA localization and translational regulation ensures that Oskar is only expressed at the proper location. One key regulatory factor is Bruno, which represses translation of oskar mRNA before its localization. Ectopic expression of a bruno cDNA prolongs repression, even after oskar mRNA is localized, and posterior body patterning is efficiently and selectively blocked. Surprisingly, the initial accumulation of Oskar, while frequently reduced, is not eliminated, arguing that levels of Oskar previously thought to be sufficient for patterning do not suffice, or that Bruno acts at a downstream step in patterning. Expression of the bruno cDNA does not inhibit posterior patterning when Oskar is expressed independent of Bruno-mediated regulation, ruling out a downstream requirement for Bruno. Notably, an Oskar::GFP reporter protein reveals continual accumulation during the late phases of oogenesis. Taken together, these results strongly argue that a late phase in accumulation of Osk protein, typically not monitored because of imperviousness of late stage oocytes to antibodies, is crucial for body patterning.

A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. Publishing Authors By Initials

MJ Snee,D Harrison,N Yan,PM Macdonald,

For similar peptides: intercellular signaling peptides and proteins: transforming growth factors: transforming growth factor alpha research abstracts see: peptides: intercellular signaling peptides and proteins: transforming growth factors: transforming growth factor alpha research

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A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Differentiation; research in biological diversity

VOLUME: 75

Page Numbers: 246-55

Journal Abbreviation: Differentiation

ISSN: 0301-4681

DAY: 3

MONTH: Mar

YEAR: 2007

A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. Information

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LANGUAGE: eng

NlmUniqueID: 401650

A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor alpha

MESH TERMS: metabolism

Chemical & Substance for Abstract: A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno.

AFFILIATION: Section of Molecular, Cell, and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM54490

ACRONYM: GM

MEDLINETA: Differentiation

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