A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis.

A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis. Research Abstract Details 

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A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis. Abstract Text:

Eung-Goo Lee,Seon-Hee Kim,Young-An Bae,Joon-Yong Chung,Myungkoo Suh,Byoung-Kuk Na,Tong-Soo Kim,Insug Kang,Liang Ma,Yoon Kong,Eung-Goo Lee,Seon-Hee Kim,Young-An Bae,Joon-Yong Chung,Myungkoo Suh,Byoung-Kuk Na,Tong-Soo Kim,Insug Kang,Liang Ma,Yoon Kong,

Parasitic organisms are incapable of de novo fatty acid synthesis due to a down-regulated expression of enzymes involved in the oxygen-dependent pathway. We investigated the uptake of host lipids by a 150-kDa hydrophobic ligand-binding protein (HLBP) of Taenia solium metacestode, an agent causative of neurocysticercosis. The protein was found to be a hetero-oligomeric complex consisting of multiple subunits (M(r) 7, 10, and 15 kDa within pH 8.0-9.7), which may originate from four unique genes of 7- and 10-kDa gene families with 2-3 polymorphic alleles/paralogs. The 15-kDa protein represented glycosylated forms of the 10-kDa. With high binding affinity to lipid analogs, these subunits evidenced high-level sequence identity with other cestode HLBPs and form a novel clade associated with excretory-secretory type HLBP. In vitro experiments with viable worms suggested that the excreted 150-kDa protein might bind to lipids, and participate in the translocation of host lipids across the syncytial membrane. This process was substantially inhibited by the specific anti-150 kDa antibodies. The protein was localized in the parasite syncytium and in the lipid droplets within host granuloma wall, where significant lipase activity was expressed. HLBP-mediated uptake of the host lipid may be critical for the parasite survival and thus could be targeted by chemotherapeutics and/or vaccine.

A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis. Publishing Authors By Initials

EG Lee,SH Kim,YA Bae,JY Chung,M Suh,BK Na,TS Kim,I Kang,L Ma,Y Kong,EG Lee,SH Kim,YA Bae,JY Chung,M Suh,BK Na,TS Kim,I Kang,L Ma,Y Kong,

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A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proteomics

VOLUME: 7

Page Numbers: 4016-30

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 31

MONTH: Nov

YEAR: 2007

A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis. Information

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LANGUAGE: eng

NlmUniqueID: 101092707

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Grant and Affiliation Information for A hydrophobic ligand-binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis.

AFFILIATION: Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon, Korea.

Country: Germany

AGENCY: United States PHS

GRANT: 347-6111-216

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MEDLINETA: Proteomics

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