A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes.

A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes. Research Abstract Details 

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A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes. Abstract Text:

Kimitoshi Yagami,Yohei Uyama,Yasumasa Yoshizawa,Saburo Kakuta,Akira Yamaguchi,Masao Nagumo,

We investigated the differentiation potential into various mesenchymal cell lineages of the clonal cell line (USAC) that was isolated from a human chondrogenic osteosarcoma. USAC cells produced types II and X collagens and proteoglycan, indicating chondrocyte differentiation. Production of type I collagen and osteocalcin by USAC cells demonstrated osteoblastic properties. They also differentiated into adipocytes generating numerous lipid droplets in their cytoplasm. Recombinant human bone morphogenetic protein-2 (rhBMP-2) increased production of proteoglycan, types II and X collagens and osteocalcin. This indicates that rhBMP-2 promotes both chondroblastic and osteoblastic differentiation in USAC cells. rhBMP-2 inhibited adipocyte differentiation. USAC cells transplanted with rhBMP-2 into the peritoneal cavities of athymic mice using diffusion chambers generated cartilage and bone more effectively in the diffusion chambers than those produced without rhBMP-2. Although USAC cells also produced adipose tissue in the diffusion chambers following transplantation with or without rhBMP-2, rhBMP-2 treatment reduced the amounts of adipose tissue. These results demonstrate that USAC is a suitable model to explore regulatory mechanisms involved in human mesenchymal cell differentiation.

A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes. Publishing Authors By Initials

K Yagami,Y Uyama,Y Yoshizawa,S Kakuta,A Yamaguchi,M Nagumo,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Bone

VOLUME: 34

Page Numbers: 648-55

Journal Abbreviation: Bone

ISSN: 8756-3282

DAY: 19

MONTH: Apr

YEAR: 2004

A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes. Information

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LANGUAGE: eng

NlmUniqueID: 8504048

A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: pharmacology

Chemical & Substance for Abstract: A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for A human chondrogenic cell line retains multi-potency that differentiates into osteoblasts and adipocytes.

AFFILIATION: Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Tokyo, Japan. kimi@senzoku.showa-u.ac.jp

Country: United States

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MEDLINETA: Bone

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