A HIV-2-based self-inactivating vector for enhanced gene transduction.

A HIV-2-based self-inactivating vector for enhanced gene transduction. Research Abstract Details 

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A HIV-2-based self-inactivating vector for enhanced gene transduction. Abstract Text:

Sayandip Mukherjee,Hui-Ling Rose Lee,Annmarie L Pacchia,Yacov Ron,Joseph P Dougherty,

The employment of HIV-1-based vectors in clinical trials is controversial mainly due to the lethal nature of the virus. HIV-2 is less pathogenic in nature and therefore is likely to be safer for vector design and production. We developed HIV-2-based self-inactivating vectors in which 520 bp out of 554 bp of the viral U3 was deleted. Interestingly, high titers were obtained only when an exogenous promoter was used to drive expression of viral RNA. It was found that the vectors could target a wide range of mammalian cell types including primary neuronal cells and could yield long term expression. It is also noteworthy that the HIV-2 vectors could be effectively cross-packaged into HIV-1 core, which might provide for enhanced safety by reducing the recombination potential of the system.

A HIV-2-based self-inactivating vector for enhanced gene transduction. Publishing Authors By Initials

S Mukherjee,HL Lee,AL Pacchia,Y Ron,JP Dougherty,

For similar investigative techniques: genetic techniques: gene transfer techniques: transduction, genetic research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transduction, genetic research

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A HIV-2-based self-inactivating vector for enhanced gene transduction. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of biotechnology

VOLUME: 127

Page Numbers: 745-57

Journal Abbreviation: J. Biotechnol.

ISSN: 0168-1656

DAY: 18

MONTH: 09

YEAR: 2006

A HIV-2-based self-inactivating vector for enhanced gene transduction. Information

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LANGUAGE: eng

NlmUniqueID: 8411927

A HIV-2-based self-inactivating vector for enhanced gene transduction. Keywords Mesh Terms:

KEYWORDS: Transduction, Genetic

MESH TERMS: genetics

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Grant and Affiliation Information for A HIV-2-based self-inactivating vector for enhanced gene transduction.

AFFILIATION: Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA.

Country: Netherlands

AGENCY: United States NCI

GRANT: CA50777

ACRONYM: CA

MEDLINETA: J Biotechnol

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