A high-throughput screening approach to anthrax lethal factor inhibition.

A high-throughput screening approach to anthrax lethal factor inhibition. Research Abstract Details 

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A high-throughput screening approach to anthrax lethal factor inhibition. Abstract Text:

Sherida L Johnson,Li-Hsing Chen,Maurizio Pellecchia,

A high-throughput screening approach was used to identify new inhibitors of the metallo-protease lethal factor from Bacillus anthracis. A library of approximately 14,000 compounds was screened using a fluorescence-based in vitro assay and hits were further characterized enzymatically via measurements of IC50 and Ki values against a small panel of metallo-proteases. This study led to the identification of new scaffolds that inhibit LF and the Botulinum Neurotoxin Type A in the low micromolar range, while sparing the human metallo-proteases MMP-2 and MMP-9. Therefore, these scaffolds could be further exploited for the development of potent and selective anti-toxin agents.

A high-throughput screening approach to anthrax lethal factor inhibition. Publishing Authors By Initials

SL Johnson,LH Chen,M Pellecchia,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure research

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A high-throughput screening approach to anthrax lethal factor inhibition. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Bioorganic chemistry

VOLUME: 35

Page Numbers: 306-12

Journal Abbreviation: Bioorg. Chem.

ISSN: 0045-2068

DAY: 22

MONTH: 02

YEAR: 2007

A high-throughput screening approach to anthrax lethal factor inhibition. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1303703

A high-throughput screening approach to anthrax lethal factor inhibition. Keywords Mesh Terms:

KEYWORDS: Molecular Structure

MESH TERMS: antagonists & inhibitors

Chemical & Substance for Abstract: A high-throughput screening approach to anthrax lethal factor inhibition. Information

Substance Name: Metalloproteases

Registry Number: EC 3.4.-

Grant and Affiliation Information for A high-throughput screening approach to anthrax lethal factor inhibition.

AFFILIATION: Burnham Institute for Medical Research, La Jolla, CA 92037, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI059572

ACRONYM: AI

MEDLINETA: Bioorg Chem

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