A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates.

A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates. Research Abstract Details 

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A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates. Abstract Text:

Tanya M Leavy,Carolyn R Bertozzi,

O-GlcNAc transferase (OGT) catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto a diverse array of intracellular proteins. Although hundreds of proteins are known to be modified by O-GlcNAc, a strict amino acid consensus sequence for OGT has not been identified. In this study, we describe the development of a high-throughput assay for OGT and use it to profile the specificity of the enzyme among a panel of peptide substrates.

A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates. Publishing Authors By Initials

TM Leavy,CR Bertozzi,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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MEDLINE DATE:

A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Bioorganic & medicinal chemistry letters

VOLUME: 17

Page Numbers: 3851-4

Journal Abbreviation: Bioorg. Med. Chem. Lett.

ISSN: 0960-894X

DAY: 10

MONTH: 05

YEAR: 2007

A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9107377

A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates. Information

Substance Name: O-GlcNAc transferase

Registry Number: EC 2.4.1.-

Grant and Affiliation Information for A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates.

AFFILIATION: Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM066047

ACRONYM: GM

MEDLINETA: Bioorg Med Chem Lett

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