A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology.

A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology. Abstract Text:

Suzanne S Eveland,Denise P Milot,Qiu Guo,Ying Chen,Sheryl A Hyland,Laurence B Peterson,Sylvie Jezequel-Sur,Gregory T O'Donnell,Paul D Zuck,Marc Ferrer,Berta Strulovici,John A Wagner,Wesley K Tanaka,Deborah A Hilliard,Omar Laterza,Samuel D Wright,Carl P Sparrow,Matt S Anderson,

Cholesteryl ester transfer protein (CETP) is a serum component responsible for both cholesteryl ester and triglyceride trafficking between high-density lipoprotein (HDL) and the apolipoprotein B (apoB)-containing very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL). Several fluorescence-based assays that monitor these transfers have been reported, but to date such assays have suffered from a low signal/background (S/B) ratio and have been described for use only in relatively purified in vitro systems. We have modified the more advanced of these assays to incorporate a noninterfering, nondiffusable fluorescence quencher into previously described cosonicate particles, often referred to as microemulsions. This simple improvement resulted in particles that had an average threefold enhanced S/B window over particles without quenchers but that continued to show the essential properties of a catalytic assay, including catalysis to a single endpoint, excellent linearity with protein and particle concentration, and an appropriate sensitivity to inhibition. This reduced assay noise allowed the subsequent development of protocols for the direct measure of cholesteryl ester (CE) transfer activity resident in human and animal serum as well as the development of 384- and 3456-well screening protocols with good precision and accuracy. Thus, by expanding the dynamic response window of the assay, we have created an assay generalizable to many settings.

A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology. Publishing Authors By Initials

SS Eveland,DP Milot,Q Guo,Y Chen,SA Hyland,LB Peterson,S Jezequel-Sur,GT O'Donnell,PD Zuck,M Ferrer,B Strulovici,JA Wagner,WK Tanaka,DA Hilliard,O Laterza,SD Wright,CP Sparrow,MS Anderson,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

PUBMED ID PMID:

MEDLINE DATE:

A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical biochemistry

VOLUME: 368

Page Numbers: 239-49

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 8

MONTH: 06

YEAR: 2007

A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370535

A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: methods

Chemical & Substance for Abstract: A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology. Information

Substance Name: Fluorescent Dyes

Registry Number: 0

Grant and Affiliation Information for A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology.

AFFILIATION: Department of Cardiovascular Diseases, Merck Research Laboratories, Rahway, NJ 07065, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Anal Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A high-precision fluorogenic cholesteryl ester transfer protein assay compatible with animal serum and 3456-well assay technology Related Publications

• CONENOSE BUGS (TRIATOMA) VISIT UNOCCUPIED BOY'S CAMP IN LOS ANGELES.
• Comparison of the generic neuronal differentiation and neuron subtype specification functions of mammalian achaete-scute and atonal homologs in cultured neural progenitor cells.
• Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells.
• Diet, lifestyle and chronic disease risk: Assessing and improvingstrategies for preventive interventions, a global perspective.
• Effect of dietary vitamin E on immune responses of calves.
• Effect of maternal mental health problems on child treatment response in community-based services.
• Genomic instability in invasive breast carcinoma measured by inter-Simple Sequence Repeat PCR.
• Identification of dividing, determined sensory neuron precursors in the mammalian neural crest.
• Improved reproductive performance from dairy cows treated with dinoprost tromethamine soon after calving.
• Integration of multiple instructive cues by neural crest stem cells reveals cell-intrinsic biases in relative growth factor responsiveness.
• Localization of bone morphogenetic proteins (BMPs)-2, -4, and -6 within megakaryocytes and platelets.
• MASH1 activates expression of the paired homeodomain transcription factor Phox2a, and couples pan-neuronal and subtype-specific components of autonomic neuronal identity.
• MASH1 maintains competence for BMP2-induced neuronal differentiation in post-migratory neural crest cells.
• Mechanisms and therapy for retained fetal membranes and uterine infections of cows: A review.
• Mutant K-rasV12 increases COX-2, peroxides and DNA damage in lung cells.
• Neural crest stem cells undergo cell-intrinsic developmental changes in sensitivity to instructive differentiation signals.
• Porcine malignant hyperthermia.
• Posttranscriptional mechanisms regulating the inflammatory response.
• Prevalence of postservice pyometra in a herd of beef cows infected with trichomoniasis: A case report.
• Prospective identification, isolation by flow cytometry, and in vivo self-renewal of multipotent mammalian neural crest stem cells.
• Short-term storage of rabbit embryos at 4 degrees C.
• Stewart-Treves syndrome: MR imaging of a postmastectomy upper-limb chronic lymphedema with angiosarcoma.
• Transient Notch activation initiates an irreversible switch from neurogenesis to gliogenesis by neural crest stem cells.
• Tumor cell expression of CD59 is associated with resistance to CD20 serotherapy in patients with B-cell malignancies.
• Two different centric fusion chromosomal defects in a Simbrah bull: A case report.
• Viability of blastocysts produced by aggregation of two half-embryos in the mouse.
• [Mini ecosystem summary of three flight experiment (4) performance of digital video camcorder implemented for mini ecosystem on space station Mir]
• [Space experiments on mini closed ecosystem--results from three flights--(1) water, organic compounds, carbon isotopic ratio]
• [Space experiments on mini closed ecosystem--results from three flights--(2) microorganisms and plant]
• [Space experiments on mini closed ecosystem--results from three flights--(3) animals]