A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure.

A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure. Research Abstract Details 

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A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure. Abstract Text:

Christina T Hauser,Roger Y Tsien,

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically encodable domains (<15 aa) offer complementary advantages. We introduce a small fluorescent chelator whose membrane-impermeant complex with nontoxic Zn(2+) ions binds tightly but reversibly to hexahistidine (His(6)) motifs on surface-exposed proteins. This live-cell label helps to resolve a current controversy concerning externalization of the stromal interaction molecule STIM1 upon depletion of Ca(2+) from the endoplasmic reticulum. Whereas N-terminal fluorescent protein fusions interfere with surface exposure of STIM1, short His(6) tags are accessible to the dye or antibodies, demonstrating externalization.

A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure. Publishing Authors By Initials

CT Hauser,RY Tsien,

For similar inorganic chemicals: elements: metals, heavy: zinc research abstracts see: inorganic chemicals: elements: metals, heavy: zinc research

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A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 3693-7

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 28

MONTH: 02

YEAR: 2007

A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure. Keywords Mesh Terms:

KEYWORDS: Zinc

MESH TERMS: chemistry

Chemical & Substance for Abstract: A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure.

AFFILIATION: Department of Pharmacology, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093-0647, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS 27177

ACRONYM: NS

MEDLINETA: Proc Natl Acad Sci U S A

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