A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme.

A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme. Research Abstract Details 

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A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme. Abstract Text:

Zigmund Luka,Antonieta Capdevila, Mato,C Wagner,

Three human cases having mutations in the glycine N-methyltransferase (GNMT) gene have been reported. This enzyme transfers a methyl group from S-adenosylmethionine (SAM) to glycine to form S-adenosylhomocysteine (SAH) and N-methylglycine (sarcosine) and is believed to be involved in the regulation of methylation. All three cases have mild liver disease but they seem otherwise unaffected. To study this further, gnmt deficient mice were generated for the first time. This resulted in the complete absence of GNMT protein and its activity in livers of homozygous mice. Compared to WT animals the absence of GNMT resulted in up to a 7-fold increase of free methionine and up to a 35-fold increase of SAM. The amount of SAH was significantly decreased (3 fold) in the homozygotes compared to WT. The ratio of SAM/SAH increased from 3 in WT to 300 in livers of homozygous transgenic mice. This suggests a possible significant change in methylation in the liver and other organs where GNMT is expressed.

A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme. Publishing Authors By Initials

Z Luka,A Capdevila,JM Mato,C Wagner,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: regulatory sequences, nucleic acid: promoter regions (genetics) research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: regulatory sequences, nucleic acid: promoter regions (genetics) research

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A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Transgenic research

VOLUME: 15

Page Numbers: 393-7

Journal Abbreviation: Transgenic Res.

ISSN: 0962-8819

DAY: 3

MONTH: Jun

YEAR: 2006

A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme. Information

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LANGUAGE: eng

NlmUniqueID: 9209120

A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme. Keywords Mesh Terms:

KEYWORDS: Promoter Regions (Genetics)

MESH TERMS: metabolism

Chemical & Substance for Abstract: A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme. Information

Substance Name: Glycine N-Methyltransferase

Registry Number: EC 2.1.1.20

Grant and Affiliation Information for A glycine N-methyltransferase knockout mouse model for humans with deficiency of this enzyme.

AFFILIATION: Department of Biochemistry, Vanderbilt University School of Medicine, 620 Light Hall, Nashville, TN 37232, USA. conrad.Wagner@vanderbilt.edu

Country: Netherlands

AGENCY: United States NIDDK

GRANT: DK 15289

ACRONYM: DK

MEDLINETA: Transgenic Res

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