A Genome-wide Approach to Identify the Genes Involved in Biofilm Formation in E. coli.

A Genome-wide Approach to Identify the Genes Involved in Biofilm Formation in E. coli. Research Abstract Details 

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A Genome-wide Approach to Identify the Genes Involved in Biofilm Formation in E. coli. Abstract Text:

Emma Tabe Eko Niba,Yoshiaki Naka,Megumi Nagase,Hirotada Mori,Madoka Kitakawa,

Biofilm forming cells are distinctive from the well-investigated planktonic cells and exhibit a different type of gene expression. Several new Escherichia coli genes related to biofilm formation have recently been identified through genomic approaches such as DNA microarray analysis. However, many others involved in this process might have escaped detection due to poor expression, regulatory mechanism, or genetic backgrounds. Here, we screened a collection of single-gene deletion mutants of E. coli named 'Keio collection' to identify genes required for biofilm formation. Of the 3985 mutants of non-essential genes in the collection thus examined, 110 showed a reduction in biofilm formation nine of which have not been well characterized yet. Systematic and quantitative analysis revealed the involvement of genes of various functions and reinforced the importance in biofilm formation of the genes for cell surface structures and cell membrane. Characterization of the nine mutants of function-unknown genes indicated that some of them, such as yfgA that genetically interacts with a periplasmic chaperone gene surA together with yciB and yciM, might be required for the integrity of outer membrane.

A Genome-wide Approach to Identify the Genes Involved in Biofilm Formation in E. coli. Publishing Authors By Initials

ET Niba,Y Naka,M Nagase,H Mori,M Kitakawa,

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A Genome-wide Approach to Identify the Genes Involved in Biofilm Formation in E. coli. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: DNA research : an international journal for rapid

VOLUME: 14

Page Numbers: 237-46

Journal Abbreviation: DNA Res.

ISSN: 1340-2838

DAY: 7

MONTH: 01

YEAR: 2008

A Genome-wide Approach to Identify the Genes Involved in Biofilm Formation in E. coli. Information

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LANGUAGE: eng

NlmUniqueID: 9423827

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Grant and Affiliation Information for A Genome-wide Approach to Identify the Genes Involved in Biofilm Formation in E. coli.

AFFILIATION: 1Graduate School of Science and Technology, Kobe University, Kobe, Hyogo 657-8501, Japan.

Country: England

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MEDLINETA: DNA Res

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