A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide.

A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide. Research Abstract Details 

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A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide. Abstract Text:

Takashi Morii,Tomohisa Tanaka,Shin-ichi Sato,Masaki Hagihara,Yasunori Aizawa,Keisuke Makino,

Short peptides could potentially provide a novel element to read-out DNA sequences from the major groove. However, it is difficult to determine sequence-preference of de novo designed monomeric short peptides. Because DNS-binding affinity and specificity of short peptides are usually much lower than those of native DNA-binding proteins, determining the sequence-preference of short peptides by conventional methods utilized to deduce the target sequence of proteins often produces an unclear outcome. We report here a general strategy to defining the sequence-preference of a DNA-binding short peptide by using the heterodimers. A GCN4 basic region peptide tethers a low-affinity DNA-binding peptide adjacent to a GCN4 binding sequence through the cyclodextrin-adamantane association, thereby increasing local concentration of the low-affinity peptide on degenerated DNA sequences. An increase of the local concentration allows one to select a preferential sequence for the low-affinity DNA binding peptide. The method successfully identified specific sequences of short peptides derived from native DNA-binding proteins. The usefulness of this approach has been demonstrated by identifying preferred DNA targets for a peptide composed only of d-amino acids. The method is potentially applicable not only to artificial peptides, but also to other synthethic ligands.

A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide. Publishing Authors By Initials

T Morii,T Tanaka,S Sato,M Hagihara,Y Aizawa,K Makino,

For similar proteins: fungal proteins: saccharomyces cerevisiae proteins research abstracts see: proteins: fungal proteins: saccharomyces cerevisiae proteins research

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A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 124

Page Numbers: 180-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 16

MONTH: Jan

YEAR: 2002

A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide. Information

Substance Name: Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for A general strategy to determine a target DNA sequence of a short peptide: application to a d-peptide.

AFFILIATION: Institute of Advanced Energy, Kyoto University, Japan. t-morii@iae.kyoto-u.ac.jp

Country: United States

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MEDLINETA: J Am Chem Soc

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