A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins.

A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Research Abstract Details 

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A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Abstract Text:

Yingqi Xu,Zhi Lin,Chien Ho,Daiwen Yang,

A general strategy is proposed to assign aliphatic side-chain resonances of large 13C,15N-labeled proteins without deuteration, using 4D 13C,15N-edited NOESY and MQ-(H)CCH-TOCSY experiments on the basis of prior assignments of backbone and 13Cbeta resonances. The strategy has been tested on a 214 residue protein (DdCAD-1) and applied to a chain-selectively 13C,15N-labeled hemoglobin (65 kDa). About 96 and 80% aliphatic side-chain spins in DdCAD-1 and hemoglobin have been assigned, respectively. The strategy proposed here will be very useful for the structure determination and dynamics characterization of large proteins by NMR.

A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Publishing Authors By Initials

Y Xu,Z Lin,C Ho,D Yang,

For similar proteins research abstracts see: proteins research

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A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 11920-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 31

MONTH: Aug

YEAR: 2005

A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins.

AFFILIATION: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543.

Country: United States

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MEDLINETA: J Am Chem Soc

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