A general purpose method for extracting RNA from Dictyostelium cells.

A general purpose method for extracting RNA from Dictyostelium cells. Research Abstract Details 

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A general purpose method for extracting RNA from Dictyostelium cells. Abstract Text:

Karen E Pilcher,Pascale Gaudet,Petra Fey,Anthony S Kowal,Rex L Chisholm,Karen E Pilcher,Pascale Gaudet,Petra Fey,Anthony S Kowal,Rex L Chisholm,

Here we present a protocol for the extraction of RNA from Dictyostelium discoideum. Dictyostelium is a social amoeba that undergoes a basic developmental program, and therefore analysis of RNA levels over a time course is a commonly used technique. This procedure is similar to other guanidine thiocyanate-based methods; however, it has been adjusted because of the large quantities of carbohydrate and nucleases found in Dictyostelium cells. After cell lysis and phenol:chloroform extraction, the resulting high-quality RNA isolated with the described protocol allows the molecular genetic analysis of wild-type and genetically modified cells. The purified RNA can be used for analyses such as northern blotting, RT-PCR and microarrays. This procedure requires approximately 2 h to complete.

A general purpose method for extracting RNA from Dictyostelium cells. Publishing Authors By Initials

KE Pilcher,P Gaudet,P Fey,AS Kowal,RL Chisholm,KE Pilcher,P Gaudet,P Fey,AS Kowal,RL Chisholm,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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A general purpose method for extracting RNA from Dictyostelium cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature protocols

VOLUME: 2

Page Numbers: 1329-32

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

A general purpose method for extracting RNA from Dictyostelium cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

A general purpose method for extracting RNA from Dictyostelium cells. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: A general purpose method for extracting RNA from Dictyostelium cells. Information

Substance Name: Chloroform

Registry Number: 67-66-3

Grant and Affiliation Information for A general purpose method for extracting RNA from Dictyostelium cells.

AFFILIATION: dictyBase, Center for Genetic Medicine, Northwestern University, 676 North Saint Clair Street Suite 1260, Chicago, Illinois 60611, USA.

Country: England

AGENCY: United States NHGRI

GRANT: HG00022

ACRONYM: HG

MEDLINETA: Nat Protoc

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