A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality.

A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. Research Abstract Details 

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A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. Abstract Text:

Nicholas A Salmon,Renee A Reijo Pera,Eugene Yujun Xu,

Outer dense fiber 2 (Odf2) is highly expressed in the testis where it encodes a major component of the outer dense fibers of the sperm flagellum. Furthermore, ODF2 protein has recently been identified as a widespread centrosomal protein. While the expression of Odf2 highlighted a potential role for this gene in male germ cell development and centrosome function, the in vivo function of Odf2 was not known. We have generated Odf2 knockout mice using an Odf2 gene trapped embryonic stem cell (ESC) line. Insertion of a gene trap vector into exon 9 resulted in a gene that encodes a severely truncated protein lacking a large portion of its predicted coil forming domains as well as both leucine zipper motifs that are required for protein-protein interactions with ODF1, another major component of the outer dense fibers. Although wild-type and heterozygous mice were recovered, no mice homozygous for the Odf2 gene trap insertion were recovered in an extended breeding program. Furthermore, no homozygous embryos were found at the blastocyst stage of embryonic development, implying a critical pre-implantation role for Odf2. We show that Odf2 is expressed widely in adults and is also expressed in the blastocyst stage of preimplantation development. These findings are in contrast with early studies reporting Odf2 expression as testis specific and suggest that embryonic Odf2 expression plays a critical role during preimplantation development in mice.

A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. Publishing Authors By Initials

NA Salmon,RA Reijo Pera,EY Xu,

For similar diagnosis: laboratory techniques and procedures: sperm count research abstracts see: diagnosis: laboratory techniques and procedures: sperm count research

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A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genesis (New York, N.Y. : 2000)

VOLUME: 44

Page Numbers: 515-22

Journal Abbreviation: Genesis

ISSN: 1526-954X

DAY: 3

MONTH: Nov

YEAR: 2006

A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100931242

A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. Keywords Mesh Terms:

KEYWORDS: Sperm Count

MESH TERMS: genetics

Chemical & Substance for Abstract: A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. Information

Substance Name: Odf2 protein, mouse

Registry Number: 0

Grant and Affiliation Information for A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality.

AFFILIATION: Center for Reproductive Sciences, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California-San Francisco, San Francisco, California, USA.

Country: United States

AGENCY: United States NICHD

GRANT: U01 HD045871

ACRONYM: HD

MEDLINETA: Genesis

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