A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins.

A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins. Research Abstract Details 

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A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins. Abstract Text:

Daniel Abankwa,Horst Vogel,

The standard model of heterotrimeric G protein signaling postulates a dissociation of Galpha and Gbetagamma subunits after activation. We hypothesized that the different combination of lipid-modifications on Galpha and Galphabetagamma subunits directs them into different microdomains. By characterizing rapidly and at high sensitivity 38 fluorescence resonance energy transfer (FRET) pairs of heterotrimeric-G-protein constructs, we defined their microdomains in relation to each other, free from the constraints of the raft/non-raft dualism. We estimated that in a cell approximately 30% of these membrane-anchored proteins are mostly clustered in 3400-16,200 copies of 30-nm microdomains. We found that the membrane anchors of Galpha and Galphabetagamma subunits of both the G(i/o) and G(q) family co-cluster differently with microdomain markers. Moreover, anchors of the Galpha(i/o) and Galpha(q) subunits co-clustered only weakly, whereas constructs that contained the anchors of the corresponding heterotrimers co-clustered considerably, suggesting the existence of at least three types of microdomain. Finally, FRET experiments with full-length heterotrimeric G proteins confirmed that the inactive, heterotrimerized Galpha subunit is in microdomains shared by heterotrimers from different subclasses, from where it displaces upon activation into a membrane-anchor- and subclass-specific microdomain.

A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins. Publishing Authors By Initials

D Abankwa,H Vogel,

For similar investigative techniques: epidemiologic methods: epidemiologic research design: reproducibility of results research abstracts see: investigative techniques: epidemiologic methods: epidemiologic research design: reproducibility of results research

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A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of cell science

VOLUME: 120

Page Numbers: 2953-62

Journal Abbreviation: J. Cell. Sci.

ISSN: 0021-9533

DAY: 15

MONTH: Aug

YEAR: 2007

A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins. Information

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LANGUAGE: eng

NlmUniqueID: 52457

A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins. Keywords Mesh Terms:

KEYWORDS: Reproducibility of Results

MESH TERMS: metabolism

Chemical & Substance for Abstract: A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins. Information

Substance Name: Heterotrimeric GTP-Binding Proteins

Registry Number: EC 3.6.1.46

Grant and Affiliation Information for A FRET map of membrane anchors suggests distinct microdomains of heterotrimeric G proteins.

AFFILIATION: Ecole Polytechnique Fédérale de Lausanne (EPFL), Institut des Sciences et Ingénierie Chimiques, CH-1015 Lausanne, Switzerland. d.abankwa@imb.uq.edu.au

Country: England

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MEDLINETA: J Cell Sci

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