A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection.

A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection. Research Abstract Details 

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A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection. Abstract Text:

Mark J Pilgrim,Laura Kasman,Jasvir Grewal,Mary E Bruorton,Phil Werner,Lucille London,Steven D London,

While the salivary gland has been recognized as an important effector site of the common mucosal immune system, a useful model for studying anti-viral salivary gland immune responses in vivo and for exploring the role of the salivary gland within the common mucosal system has been lacking. Murine cytomegalovirus (MCMV) is a beta-herpesvirus that displays a strong tropism for the salivary gland and produces significant morbidity in susceptible mice when introduced by intraperitoneal (i.p.) inoculation. This study tested the hypothesis that MCMV morbidity and pathology could be reduced by injecting the virus directly the submandibular salivary gland (intraglandular (i.g.)), using either in vivo derived MCMV or the less virulent, tissue-culture-derived MCMV (tcMCMV). Peak salivary gland viral titers were completely unaffected by infection route (i.p vs. i.g.) after inoculation with either MCMV or tcMCMV. However, i.g. tcMCMV inoculation reduced viremia in all systemic tissues tested compared to i.p. inoculation. Furthermore, systemic organ pathology observed in the liver and spleen after i.p. inoculation with either MCMV or tcMCMV was completely eliminated by i.g. inoculation with tcMCMV. Cellular infiltrates in the salivary glands, after i.p. or i.g. inoculation were composed of both B and T cells, indicating the potential for a local immune response to occur in the salivary gland. These results demonstrate that a focused MCMV infection of the salivary gland without systemic organ pathology is possible using i.g. delivery of tcMCMV.

A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection. Publishing Authors By Initials

MJ Pilgrim,L Kasman,J Grewal,ME Bruorton,P Werner,L London,SD London,

For similar virus diseases: viremia research abstracts see: virus diseases: viremia research

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A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Experimental and molecular pathology

VOLUME: 82

Page Numbers: 269-79

Journal Abbreviation: Exp. Mol. Pathol.

ISSN: 0014-4800

DAY: 12

MONTH: 01

YEAR: 2007

A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection. Information

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LANGUAGE: eng

NlmUniqueID: 370711

A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection. Keywords Mesh Terms:

KEYWORDS: Viremia

MESH TERMS: virology

Chemical & Substance for Abstract: A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection. Information

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Grant and Affiliation Information for A focused salivary gland infection with attenuated MCMV: an animal model with prevention of pathology associated with systemic MCMV infection.

AFFILIATION: Department of Microbiology and Immunology, Medical University of South Carolina, PO Box 250504, 173 Ashley Avenue, Charleston, SC 29425, USA.

Country: United States

AGENCY: United States NCRR

GRANT: C06 RR015455

ACRONYM: RR

MEDLINETA: Exp Mol Pathol

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