A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay.

A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay. Research Abstract Details 

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A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay. Abstract Text:

M Takahashi,K Asada,

Pulse-generation of O2- by a flash was used to determine the reactivity of O2-, O2- was produced within 10 ms by a flash of light through the excitation of FMN in the presence of N,N,N',N'-tetramethylethylenediamine and oxygen. Kinetic analysis of cytochrome c reduction by O2- generated by flash yielded the reaction rate constant between cytochrome c and O2- and the spontaneous disproportionation rate constant of O2-. We applied it for superoxide dismutase assay using a linear relation between superoxide dismutase concentration and the apparent rate constant of cytochrome c reduction by O2-. The catalytic rate constant and activation energy at pH 7.3 of bovine liver Cu,Zn-superoxide dismutase were found to be 1.75 x 10(9) M-1 . s-1 at 25 degrees C and 26.9 kJ . M-1, respectively. The kinetics of O2- decay can be also monitored at 240 nm in this flash-photometric system and gave the spontaneous disproportionation rate constant of O2- and the catalytic rate constant of superoxide dismutase.

A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay. Publishing Authors By Initials

M Takahashi,K Asada,

For similar inorganic chemicals: electrolytes: ions: anions: oxides: peroxides: superoxides research abstracts see: inorganic chemicals: electrolytes: ions: anions: oxides: peroxides: superoxides research

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A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 91

Page Numbers: 889-96

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1982

A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay. Keywords Mesh Terms:

KEYWORDS: Superoxides

MESH TERMS: analysis

Chemical & Substance for Abstract: A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay. Information

Substance Name: Superoxide Dismutase

Registry Number: EC 1.15.1.1

Grant and Affiliation Information for A flash-photometric method for determination of reactivity of superoxide: application to superoxide dismutase assay.

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Country: JAPAN

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MEDLINETA: J Biochem

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