A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector.

A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector. Abstract Text:

Ninita H Brown,Hana M Dobrovolny,Daniel J Gauthier,Patrick D Wolf,

Optical fiber-based mapping systems are used to record the cardiac action potential (AP) throughout the myocardium. The optical AP contains a contraction-induced motion artifact (MA), which makes it difficult to accurately measure the action potential duration (APD). MA is removed by preventing contraction with electrical-mechanical uncoupling drugs, such as 2,3-butanedione monoxime (BDM). We designed a novel fiber-based ratiometric optical channel using a blue light emitting diode, a diffraction grating, and a split photodetector that can accurately measure the cardiac AP without the need for BDM. The channel was designed based on simulations using the optical design software ZEMAX. The channel has an electrical bandwidth of 150 Hz and an root mean-square dark noise of 742 muV. The channel successfully recorded the cardiac AP from the wall of five rabbit heart preparations without the use of BDM. After 20-point median filtering, the mean signal/noise ratio was 25.3 V/V. The APD measured from the base of a rabbit heart was 134 +/- 8.4 ms, compared to 137.6 +/- 3.3 ms from simultaneous microelectrode recordings. This difference was not statistically significant (p-value = 0.3). The quantity of MA removed was also measured using the motion ratio. The reduction in MA was significant (p-value = 0.0001). This fiber-based system is the first of its kind to enable optical APD measurements in the beating heart wall without the use of BDM.

A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector. Publishing Authors By Initials

NH Brown,HM Dobrovolny,DJ Gauthier,PD Wolf,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

PUBMED ID PMID:

MEDLINE DATE:

A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biophysical journal

VOLUME: 93

Page Numbers: 254-63

Journal Abbreviation: Biophys. J.

ISSN: 0006-3495

DAY: 6

MONTH: 04

YEAR: 2007

A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370626

A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: methods

Chemical & Substance for Abstract: A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector.

AFFILIATION: Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA. ninita.brown@duke.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL072831-02S1

ACRONYM: HL

MEDLINETA: Biophys J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector Related Publications

• A fiber-based ratiometric optical cardiac mapping channel using a diffraction grating and split detector.
• Analysis and quantitation of NF-kappaB nuclear translocation in tumor necrosis factor alpha (TNF-alpha) activated vascular endothelial cells.
• Anhedonia in schizophrenia.
• Application of a new segmentation tool based on interactive simplex meshes to cardiac images and pulmonary MRI data.
• Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe.
• Evaluation of the vervet (Clorocebus aethiops) as a model for the assisted reproductive technologies.
• Expected net present value of pure and mixed sexed semen artificial insemination strategies in dairy heifers.
• First-trimester placental protein 13 screening for preeclampsia and intrauterine growth restriction.
• Fluorescence correlation spectroscopy: molecular complexing in solution and in living cells.
• Frontal recess anatomy study by endoscopic dissection in cadavers.
• Generation of ENU-induced mouse mutants with hypocholesterolemia: novel tools for dissecting plasma lipoprotein homeostasis.
• Introduction to IEEE-EMBS -- What Can the Society Do for You?
• Isolation and characterization of novel rhesus monkey embryonic stem cell lines.
• Metabolic response to injury and role of anabolic hormones.
• Methylation status of imprinting centers for H19/IGF2 and SNURF/SNRPN in primate embryonic stem cells.
• Nuclear transfer in nonhuman primates.
• Peripheral and cerebral atherothrombosis and cardiovascular events in different vascular territories: insights from the Framingham Study.
• Plasma phosphatidylcholine docosahexaenoic acid content and risk of dementia and Alzheimer disease: the Framingham Heart Study.
• Postsynaptic 5-HT1A receptor stimulation increases motor activity in the 6-hydroxydopamine-lesioned rat: implications for treating Parkinson's disease.
• Prognostic Factors Including Proliferation Markers Ki-67, bax, and bcl-2 in Temporal Bone Paraganglioma.
• Reduced age-related cataracts among elderly persons who reach age 90 with preserved cognition: a biomarker of successful aging?
• Reduction of interindividual-intergroup discontinuity: the role of leader accountability and proneness to guilt.
• Serum alpha-tocopherol levels after high-dose enteral vitamin E administration in patients with acute respiratory failure.
• State-by-state guide for special licensure requirements.
• The cytoplasmic Hsp70 chaperone machinery subjects misfolded and endoplasmic reticulum import-incompetent proteins to degradation via the ubiquitin-proteasome system.
• The laterodorsal tegmentum contributes to behavioral sensitization to amphetamine.
• Transcriptional profiling of rhesus monkey embryonic stem cells.
• Understanding the role of genetics in disability insurance.
• Vitamin D in patients with kidney disease: cautiously optimistic.
• [Subtype and sequence analysis of the C2-V3 region of gp120 genes among human immunodeficiency virus infected IDUs in Ruili epidemic area of Yunnan Province of China]