A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein.

A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein. Research Abstract Details 

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A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein. Abstract Text:

Takayuki K Nemoto,Yutaka Fukuma,Hideaki Itoh,Takashi Takagi,Toshio Ono,

The 70-kDa heat shock protein (Hsp70) is predominantly present intracellularly as a monomer, but a small population is converted to dimers and oligomers under certain conditions. In the present study, we investigated the dimeric structure of human inducible Hsp70. As reported earlier, the C-terminal client-binding domain (amino acids 382-641) was required for the dimerization. A 40-amino acid deletion in the client-binding domain from either the N-terminus or C-terminus greatly enhanced the dimerization potential of Hsp70. Limited proteolysis indicated that the dimer formed through truncation from the C-terminus had a conformation similar to that of the non-truncated form. Truncation experiments demonstrated that the client-binding sub-domain (amino acids 382-520) with its adjacent region up to amino acid 541 was not sufficient for the dimerization but that the region up to amino acid 561 was sufficient. Interestingly, the dimer formed through truncation from the C-terminus acquired a homomeric disulfide bridge at Cys574.

A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein. Publishing Authors By Initials

TK Nemoto,Y Fukuma,H Itoh,T Takagi,T Ono,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 139

Page Numbers: 677-87

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 2006

A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein. Information

Substance Name: Cysteine

Registry Number: 52-90-4

Grant and Affiliation Information for A disulfide bridge mediated by cysteine 574 is formed in the dimer of the 70-kDa heat shock protein.

AFFILIATION: Division of Oral Molecular Biology, Department of Developmental and Reconstructive Medicine, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588. tnemoto@net.nagasaki-u.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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