A distinctive alveolar macrophage activation state induced by cigarette smoking.

A distinctive alveolar macrophage activation state induced by cigarette smoking. Research Abstract Details 

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A distinctive alveolar macrophage activation state induced by cigarette smoking. Abstract Text:

RATIONALE: Macrophages are believed to play a central role in emphysema based largely on data from mouse models. However, the relevance of these models to smoking-related lung disease in humans is uncertain. OBJECTIVES: We sought to comprehensively characterize the effects of smoking on gene expression in human alveolar macrophages and to compare these with effects seen in transgenic mouse models of emphysema. METHODS: We used DNA microarrays with genomewide coverage to analyze alveolar macrophages from 15 smokers, 15 nonsmokers, and 15 subjects with asthma (disease control). Selected gene expression changes were validated by polymerase chain reaction and ELISA. Expression changes were compared with those identified by microarray analysis of interleukin-13-overexpressing and integrin-beta6-deficient mice, which both develop emphysema. MEASUREMENTS AND MAIN RESULTS: All 15 smokers shared a common pattern of macrophage gene expression that distinguished them from nonsmokers, a finding not observed in subjects with asthma. We identified 110 genes as differentially expressed in smokers despite using conservative statistical methods. Matrix metalloproteinase 12, a proteinase that plays a critical role in mouse models, was the third most highly induced gene in smokers (ninefold, p < 0.0001). However, most changes in smokers were not reflected in mouse models. One such finding was increased osteopontin expression in smokers (fourfold, p = 0.006), which was confirmed at the protein level and correlated with the degree of airway obstruction. CONCLUSIONS: Smoking induces a remarkably consistent and distinctive pattern of alveolar macrophage activation. These studies identify aspects of mouse models that are directly relevant to human smokers and also reveal novel potential mediators of smoking-related diseases.

A distinctive alveolar macrophage activation state induced by cigarette smoking. Publishing Authors By Initials

For similar behavior and behavior mechanisms: behavior: habits: smoking research abstracts see: behavior and behavior mechanisms: behavior: habits: smoking research

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A distinctive alveolar macrophage activation state induced by cigarette smoking. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of respiratory and critical care

VOLUME: 172

Page Numbers: 1383-92

Journal Abbreviation: Am. J. Respir. Crit. Care Med.

ISSN: 1073-449X

DAY: 15

MONTH: 09

YEAR: 2005

A distinctive alveolar macrophage activation state induced by cigarette smoking. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9421642

A distinctive alveolar macrophage activation state induced by cigarette smoking. Keywords Mesh Terms:

KEYWORDS: Smoking

MESH TERMS: metabolism

Chemical & Substance for Abstract: A distinctive alveolar macrophage activation state induced by cigarette smoking. Information

Substance Name: Matrix Metalloproteinase 12

Registry Number: EC 3.4.24.65

Grant and Affiliation Information for A distinctive alveolar macrophage activation state induced by cigarette smoking.

AFFILIATION: UCSF-Mission Bay Rock Hall, Room 545, 1550 4th Street, San Francisco, CA 94158, USA. laura.koth@ucsf.edu

Country: United States

AGENCY: United States NCRR

GRANT: RR17002

ACRONYM: RR

MEDLINETA: Am J Respir Crit Care Med

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