A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes.

A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes. Research Abstract Details 

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A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes. Abstract Text:

Yasukazu Nakahata,Mayumi Yoshida,Atsuko Takano,Haruhiko Soma,Takuro Yamamoto,Akio Yasuda,Toru Nakatsu,Toru Takumi,

BACKGROUND: The circadian expression of the mammalian clock genes is based on transcriptional feedback loops. Two basic helix-loop-helix (bHLH) PAS (for Period-Arnt-Sim) domain-containing transcriptional activators, CLOCK and BMAL1, are known to regulate gene expression by interacting with a promoter element termed the E-box (CACGTG). The non-canonical E-boxes or E-box-like sequences have also been reported to be necessary for circadian oscillation. RESULTS: We report a new cis-element required for cell-autonomous circadian transcription of clock genes. This new element consists of a canonical E-box or a non-canonical E-box and an E-box-like sequence in tandem with the latter with a short interval, 6 base pairs, between them. We demonstrate that both E-box or E-box-like sequences are needed to generate cell-autonomous oscillation. We also verify that the spacing nucleotides with constant length between these 2 E-elements are crucial for robust oscillation. Furthermore, by in silico analysis we conclude that several clock and clock-controlled genes possess a direct repeat of the E-box-like elements in their promoter region. CONCLUSION: We propose a novel possible mechanism regulated by double E-box-like elements, not to a single E-box, for circadian transcriptional oscillation. The direct repeat of the E-box-like elements identified in this study is the minimal required element for the generation of cell-autonomous transcriptional oscillation of clock and clock-controlled genes.

A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes. Publishing Authors By Initials

Y Nakahata,M Yoshida,A Takano,H Soma,T Yamamoto,A Yasuda,T Nakatsu,T Takumi,

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A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC molecular biology

VOLUME: 9

Page Numbers: 1

Journal Abbreviation: BMC Mol. Biol.

ISSN: 1471-2199

DAY: 4

MONTH: 01

YEAR: 2008

A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes. Information

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LANGUAGE: eng

NlmUniqueID: 100966983

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AFFILIATION: Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan. yasu_nakahata@yahoo.co.jp

Country: England

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MEDLINETA: BMC Mol Biol

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