A cross-linking study on the particle species of human plasma high density lipoproteins.

A cross-linking study on the particle species of human plasma high density lipoproteins. Research Abstract Details 

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A cross-linking study on the particle species of human plasma high density lipoproteins. Abstract Text:

Y Yachida,O Minari,

The present investigation was on the particle species of human plasma high density lipoprotein (HDL) characterized by the stoichiometry of their apoprotein components. HDL2-1, HDL2-2, HDL3-1, and HDL3-2 isolated from normal human plasma by sequential ultracentrifugal flotation were further subfractionated by Bio Gel A-5m gel chromatography or hydroxyapatite column chromatography, and three distinct subfractions were obtained. Subfraction 1 was obtained from all the HDL fractions and it contained mostly apolipoprotein A-I (A-I). Subfraction 2 was obtained from HDL2-2 and HDL3-1 and it contained A-I and apolipoprotein A-II (A-II) in the molar ratio of one to one, and subfraction 3 from HDL2-2 and HDL3-1 contained A-I and apolipoprotein C (C). Each subfraction was treated with bifunctional cross-linking reagents, and the intraparticle cross-linked products of apolipoproteins were examined by SDS-polyacrylamide gel electrophoresis. The results of the cross-linking studies indicated that the HDL2 fraction consisted mainly of lipoprotein particles of the (A-I)4 type and a few of the (A-I)5, (A-I)2(A-II)2, and (A-I)4(C)2 types, and that the HDL3 fraction consisted mainly of (A-I)2(A-II)2 type particles and a few (A-I)4, (A-I)3, (A-I)2, (A-I), and (A-I)3(C)2 type particles. From the results of analyses of the lipid components in the HDL of each type, it was suggested that the function of the particle species of the (A-I)n type (n = 1--5), which contained more cholesteryl ester than the (A-I)2(A-II)2 type, was concerned mainly with cholesterol metabolism.

A cross-linking study on the particle species of human plasma high density lipoproteins. Publishing Authors By Initials

Y Yachida,O Minari,

For similar natural sciences: chemistry: chemistry, physical: particle size research abstracts see: natural sciences: chemistry: chemistry, physical: particle size research

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A cross-linking study on the particle species of human plasma high density lipoproteins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 459-71

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1983

A cross-linking study on the particle species of human plasma high density lipoproteins. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A cross-linking study on the particle species of human plasma high density lipoproteins. Keywords Mesh Terms:

KEYWORDS: Particle Size

MESH TERMS: classification

Chemical & Substance for Abstract: A cross-linking study on the particle species of human plasma high density lipoproteins. Information

Substance Name: Lipoproteins, HDL

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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