A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization.

A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization. Abstract Text:

Libin Liu,Paul F Pilch,

Cavin (PTRF) has been shown to be a highly abundant protein component of caveolae, but its functional role there is unknown. Here, we confirm that cavin co-localizes with caveolin-1 in adipocytes by confocal microscopy and co-distributes with caveolin-1 in lipid raft fractions by sucrose gradient flotation. However, cavin does not directly associate with caveolin-1 as solubilization of caveolae disrupts their interaction. Cholesterol depletion with beta-cyclodextrin causes a significant down-regulation of cavin from plasma membrane lipid raft fractions. Overexpression of cavin in HEK293-Cav-1 cells and knockdown of cavin in 3T3-L1 adipocytes enhances and diminishes caveolin-1 levels, respectively, indicating an important role for cavin in maintaining the level of caveolin-1. A truncated form of cavin, eGFP-cavin-1-322, which lacks 74 amino acids from the C-terminal, reveals a microtubular network localization by confocal microscopy. Disruption of cytoskeletal elements with latrunculin B or nocodazole diminishes cavin expression without affecting the caveolin-1 amount. We propose that the presence of cavin on the inside surface of caveolae stabilizes these structures, probably through interaction with the cytoskeleton, and cavin therefore plays an important role in caveolae formation and organization.

A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization. Publishing Authors By Initials

L Liu,PF Pilch,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 283

Page Numbers: 4314-22

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 3

MONTH: 12

YEAR: 2007

A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization.

AFFILIATION: Department of Biochemistry, Boston University Medical School, Boston, Massachusetts 02118.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A critical role of cavin polymerase I and transcript release factor in caveolae formation and organization Related Publications

• "Immune" RNA--a new model for the immunotherapy of cancer.
• A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization.
• Defining the molecular forces that determine the impact of neomycin on bacterial protein synthesis: importance of the 2'-amino functionality.
• Deletion of Cavin/PTRF causes global loss of caveolae, dyslipidemia, and glucose intolerance.
• Filoviruses: recent advances and future challenges.
• Isolation of GLUT4 Storage Vesicles.
• Mediation of immune responses to tumor antigens by immune RNA.
• Nur77 coordinately regulates expression of genes linked to glucose metabolism in skeletal muscle.
• Role of insulin-dependent cortical fodrin/spectrin remodeling in glucose transporter 4 translocation in rat adipocytes.
• The interaction of Akt with APPL1 is required for insulin-stimulated Glut4 translocation.
• The mass action hypothesis: formation of Glut4 storage vesicles, a tissue-specific, regulated exocytic compartment.
• Topoisomerase I-mediated DNA cleavage induced by the minor groove-directed binding of bibenzimidazoles to a distal site.
• [A CASE OF RETROPERITONEAL TUMOR IN A PATIENT WITH NEUROFIBROMATOSIS CUTIS (RECKLINGHAUSEN'S DISEASE).]
• [Changes in the nasal and pharyngeal mucosa in persons exposed to cement dust]
• [Complication after laser assisted uvulopalatoplasty in the treatment of snoring and obstructive sleep apnea syndrome]
• [Histologic changes in the nasopharyngeal mucosa in experimental animals exposed to cement dust]
• [Intracellular enzymatic reactivity of lymphocytes and neutrophils in patients with laryngeal carcinoma]
• [Lysosomal lymphocytic acid phophatase in patients with laryngeal carcinoma]
• [Prolapse of the everted cecum through a fecal fistula as a complication of incarcerated right-sided scrotal hernia]