A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds.

A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds. Research Abstract Details 

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A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds. Abstract Text:

Peng Yu,Bo Liu,Thomas Kodadek,Peng Yu,Bo Liu,Thomas Kodadek,

We describe here a convenient procedure for assessing the relative cell permeability of chemical compounds. The assay can be used in a high-throughput mode and is particularly applicable for the evaluation of the relative permeability of compounds in a combinatorial library. The compound of interest is conjugated to a dexamethasone derivative. The entry of the conjugate into living mammalian cells triggers the nuclear transport of a Gal4 DNA binding domain-glucocorticoid receptor ligand binding domain-VP16 activation domain fusion protein and, consequently, the activation of a Gal4-responsive luciferase reporter gene. The relative cell permeability is thus described quantitatively by the level of luciferase expression. The experiments take only a few days once chemical synthesis and conjugation are finished.

A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds. Publishing Authors By Initials

P Yu,B Liu,T Kodadek,P Yu,B Liu,T Kodadek,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature protocols

VOLUME: 2

Page Numbers: 23-30

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds. Information

Substance Name: Luciferases

Registry Number: EC 1.13.12.-

Grant and Affiliation Information for A convenient, high-throughput assay for measuring the relative cell permeability of synthetic compounds.

AFFILIATION: Division of Translational Research, Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9185, USA.

Country: England

AGENCY: United States NHLBI

GRANT: N01-HV-28185

ACRONYM: HV

MEDLINETA: Nat Protoc

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