A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin.

A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin. Research Abstract Details 

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A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin. Abstract Text:

Erica Andersen-Nissen,Kelly D Smith,Richard Bonneau,Roland K Strong,Alan Aderem,

The molecular basis for Toll-like receptor (TLR) recognition of microbial ligands is unknown. We demonstrate that mouse and human TLR5 discriminate between different flagellins, and we use this difference to map the flagellin recognition site on TLR5 to 228 amino acids of the extracellular domain. Through molecular modeling of the TLR5 ectodomain, we identify two conserved surface-exposed regions. Mutagenesis studies demonstrate that naturally occurring amino acid variation in TLR5 residue 268 is responsible for human and mouse discrimination between flagellin molecules. Mutations within one conserved surface identify residues D295 and D367 as important for flagellin recognition. These studies localize flagellin recognition to a conserved surface on the modeled TLR5 structure, providing detailed analysis of the interaction of a TLR with its ligand. These findings suggest that ligand binding at the beta sheets results in TLR activation and provide a new framework for understanding TLR-agonist interactions.

A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin. Publishing Authors By Initials

E Andersen-Nissen,KD Smith,R Bonneau,RK Strong,A Aderem,

For similar genetic phenomena: variation (genetics) research abstracts see: genetic phenomena: variation (genetics) research

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A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of experimental medicine

VOLUME: 204

Page Numbers: 393-403

Journal Abbreviation: J. Exp. Med.

ISSN: 0022-1007

DAY: 5

MONTH: 02

YEAR: 2007

A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin. Information

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LANGUAGE: eng

NlmUniqueID: 2985109

A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin. Keywords Mesh Terms:

KEYWORDS: Variation (Genetics)

MESH TERMS: metabolism

Chemical & Substance for Abstract: A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin. Information

Substance Name: Flagellin

Registry Number: 12777-81-0

Grant and Affiliation Information for A conserved surface on Toll-like receptor 5 recognizes bacterial flagellin.

AFFILIATION: Institute for Systems Biology, Seattle, WA 98103, USA.

Country: United States

AGENCY: United States NIAID

GRANT: U54 AI54523

ACRONYM: AI

MEDLINETA: J Exp Med

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