A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport.

A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport. Abstract Text:

Ulrike Grosskinsky,Monika ,Michaela Fritz,Yvonne Schmid,Marina C Lamparter,Pawel Szczesny,Andrei N Lupas,Ingo B Autenrieth,Dirk Linke,Ulrike Grosskinsky,Monika ,Michaela Fritz,Yvonne Schmid,Marina C Lamparter,Pawel Szczesny,Andrei N Lupas,Ingo B Autenrieth,Dirk Linke,Ulrike Grosskinsky,Monika ,Michaela Fritz,Yvonne Schmid,Marina C Lamparter,Pawel Szczesny,Andrei N Lupas,Ingo B Autenrieth,Dirk Linke,

The Yersinia adhesin A (YadA) is a trimeric autotransporter adhesin of enteric yersiniae. It consists of three major domains: a head mediating adherence to host cells, a stalk involved in serum resistance, and an anchor that forms a membrane pore and is responsible for the autotransport function. The anchor contains a glycine residue, nearly invariant throughout trimeric autotransporter adhesins, that faces the pore lumen. To address the role of this glycine, we replaced it with polar amino acids of increasing side chain size and expressed wild-type and mutant YadA in Escherichia coli. The mutations did not impair the YadA-mediated adhesion to collagen and to host cells or the host cell cytokine production, but they decreased the expression levels and stability of YadA trimers with increasing side chain size. Likewise, autoagglutination and resistance to serum were decreased in these mutants. We found that the periplasmic protease DegP is involved in the degradation of YadA and that in an E. coli degP deletion strain, mutant versions of YadA were expressed almost to wild-type levels. We conclude that the conserved glycine residue affects both the export and the stability of YadA and consequently some of its putative functions in pathogenesis.

A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport. Publishing Authors By Initials

U Grosskinsky,M ,M Fritz,Y Schmid,MC Lamparter,P Szczesny,AN Lupas,IB Autenrieth,D Linke,U Grosskinsky,M ,M Fritz,Y Schmid,MC Lamparter,P Szczesny,AN Lupas,IB Autenrieth,D Linke,U Grosskinsky,M ,M Fritz,Y Schmid,MC Lamparter,P Szczesny,AN Lupas,IB Autenrieth,D Linke,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 9011-9

Journal Abbreviation: J. Bacteriol.

ISSN: 1098-5530

DAY: 5

MONTH: 10

YEAR: 2007

A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport.

AFFILIATION: Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Tübingen, Tübingen, Germany.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Bacteriol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport Related Publications

• A conserved glycine residue of trimeric autotransporter domains plays a key role in Yersinia adhesin A autotransport.
• A new expression system for protein crystallization using trimeric coiled-coil adaptors.
• Angiotensin-converting enzyme, enkephalinase A and aminopeptidases in the breakdown of enkephalin--studies in cell cultures.
• Antigenic difference between serum amyloid protein SSA and its low molecular weight derivative SAAL revealed by an anti-amyloid A antiserum at different temperatures.
• Changes in the redox state of pyridine nucleotides in relation to the spontaneous contractile activity of rat portal veins at elevated pH.
• Evaluation and comparison of portable emissions measurement systems and federal reference methods for emissions from a back-up generator and a diesel truck operated on a chassis dynamometer.
• Fibrillar protein deposits with tubular substructure in a systemic disease beginning as cutis laxa.
• Metal bead crystals for easy heating by direct current.
• Optimization of catalysts using specific, description-based genetic algorithms.
• Organization of projections to temporal cortex originating in the thalamic posterior intralaminar nucleus of the rat.
• Phase separation in the isolation and purification of membrane proteins.
• Sense and stretchability: The role of titin and titin-associated proteins in myocardial stress-sensing and mechanical dysfunction.
• Temperature-induced dissociation of serum amyloid protein SAA.
• Therapy of amyloid diseases.
• Thyroid Hormone Regulates Developmental Titin Isoform Transitions via the Phosphatidylinositol-3-Kinase/ AKT Pathway.
• [Blood coagulation and liver function.]
• [Clinical aspects of primary liver cancer.]
• [Clinical experiences with dominal forte, a thiophenylpyridylamine derivative.]
• [Clinical experiences with intra-tuffone in the treatment of pulmonary hemorrhage.]
• [Diagnosis and therapy of iron deficiency anemia in pregnancy]
• [Effects of phenothiazin induced therapeutic sleep in severe pain of autonomic and thalamic origin.]
• [Elective termination of respiratory therapy in amyotrophic lateral sclerosis.]
• [Experiences in the treatment of acute and chronic pain states with the phenothiazine derivatives megaphen and atosil.]
• [Functional results of radical neck dissection]
• [Hypnotics & carbohydrate metabolism.]
• [Primary and secondary dysproteinemia with purpura and with Ravnaud's disease.]
• [Progressive muscle atrophy : A rarely diagnosed variant of amyotrophic lateral sclerosis.]
• [Roentgen therapy of induratio penis plastica.]
• [Screw-in Performance of Threaded Cups.]
• [The treatment of thermolabile instruments with water after cold sterilization with Gigasept (author's transl)]