A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate.

A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Research Abstract Details 

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A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Abstract Text:

Larry A Gallagher,Elizabeth Ramage,Michael A Jacobs,Rajinder Kaul,Mitchell Brittnacher,Colin Manoil,

Francisella tularensis, the causative agent of tularemia, is one of the most infectious bacterial pathogens known and is a category A select agent. We created a sequence-defined, near-saturation transposon mutant library of F. tularensis novicida, a subspecies that causes a tularemia-like disease in rodents. The library consists of 16,508 unique insertions, an average of >9 insertions per gene, which is a coverage nearly twice that of the greatest previously achieved for any bacterial species. Insertions were recovered in 84% (1,490) of the predicted genes. To achieve high coverage, it was necessary to construct transposons carrying an endogenous Francisella promoter to drive expression of antibiotic resistance. An analysis of genes lacking (or with few) insertions identified nearly 400 candidate essential genes, most of which are likely to be required for growth on rich medium and which represent potential therapeutic targets. To facilitate genome-scale screening using the mutant collection, we assembled a sublibrary made up of two purified mutants per gene. The library provides a resource for virtually complete identification of genes involved in virulence and other nonessential processes.

A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Publishing Authors By Initials

LA Gallagher,E Ramage,MA Jacobs,R Kaul,M Brittnacher,C Manoil,

For similar genetic phenomena: variation (genetics): mutation research abstracts see: genetic phenomena: variation (genetics): mutation research

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A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 1009-14

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 10

MONTH: 01

YEAR: 2007

A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Keywords Mesh Terms:

KEYWORDS: Mutation

MESH TERMS: genetics

Chemical & Substance for Abstract: A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Information

Substance Name: DNA Transposable Elements

Registry Number: 0

Grant and Affiliation Information for A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate.

AFFILIATION: Department of Genome Sciences, University of Washington, Campus Box 355065, 1705 NE Pacific Street, Seattle, WA 98195, USA.

Country: United States

AGENCY: United States NIAID

GRANT: 5U54 AI 057141-04

ACRONYM: AI

MEDLINETA: Proc Natl Acad Sci U S A

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