A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays.

A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays. Abstract Text:

Russell S Thomas,Thomas M O'Connell,Linda Pluta,Russell D Wolfinger,Longlong Yang,Todd J Page,

Two-year rodent bioassays play a central role in evaluating the carcinogenic potential of both commercial products and environmental contaminants. The bioassays are expensive and time consuming, requiring years to complete and costing $2-4 million. In this study, we compare transcriptomic and metabonomic technologies for discovering biomarkers that can efficiently and economically identify chemical carcinogens without performing a standard two-year rodent bioassay. Animals were exposed subchronically to two chemicals (one genotoxic and one nongenotoxic) that were positive for lung and liver tumors in a standard two-year bioassay, two chemicals that were negative, and two control groups. Microarray analysis performed on liver and lung tissues identified multiple biomarkers in each tissue that could discriminate between carcinogenic and noncarcinogenic treatments. The discriminating biomarkers shared a common expression profile among carcinogenic treatments despite different genotoxicity categories and potential modes of action, suggesting that they reflect underlying cellular changes in the transition toward neoplasia. Statistical classification analysis exhibited 100% accuracy in both tissues when the number of genes was less than 5000. Additional genes reduced the predictive accuracy of the model. Serum samples were analyzed by 1H nuclear magnetic resonance (NMR) spectroscopy, and chemical-specific metabolites were removed from the spectra. The statistical classification analysis of the endogenous serum metabolites showed relatively low predictive accuracy with few metabolites in the model, but the accuracy increased to a maximum of 94% when all metabolites were added. These results suggest that individual endogenous metabolites are relatively poor biomarkers, but the metabolite profile as a whole is altered following carcinogen treatment.

A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays. Publishing Authors By Initials

RS Thomas,TM O'Connell,L Pluta,RD Wolfinger,L Yang,TJ Page,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Toxicological sciences : an official journal of th

VOLUME: 96

Page Numbers: 40-6

Journal Abbreviation: Toxicol. Sci.

ISSN: 1096-6080

DAY: 17

MONTH: 11

YEAR: 2006

A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9805461

A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: drug effects

Chemical & Substance for Abstract: A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays. Information

Substance Name: RNA, Neoplasm

Registry Number: 0

Grant and Affiliation Information for A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays.

AFFILIATION: CIIT Centers for Health Research, Research Triangle Park, North Carolina 27709-2137, USA. rthomas@ciit.org

Country: United States

AGENCY: United States NCRR

GRANT: RR000046

ACRONYM: RR

MEDLINETA: Toxicol Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays Related Publications

• 142.
• A comparison of transcriptomic and metabonomic technologies for identifying biomarkers predictive of two-year rodent cancer bioassays.
• An audit of health products and services marketed on chiropractic websites in Alberta and consideration of these practices in the context of chiropractic codes of conduct and ethics.
• Are there long-term consequences of pain in newborn or very young infants?
• Assessment of polycyclic aromatic hydrocarbon exposure in the waters of Prince William Sound after the Exxon Valdez oil spill: 1989-2005.
• Behavioral genomics of honeybee foraging and nest defense.
• Circadian regulation of insect olfactory learning.
• CpG DNA modulates interleukin 1beta-induced interleukin-8 expression in human bronchial epithelial (16HBE14o-) cells.
• Current Concepts in Menthol-based Topical Analgesics for Pain Relief: 2087: Board #157 May 31 2:00 PM - 3:30 PM.
• Effects of experimental endogenous hyperlipemia on circulating leukocytes and erythrocytes.
• Expert commentary.
• Freezing bovine embryos with a portable liquid nitrogen freezer requiring no external seeding.
• German cockroach frass proteases cleave pro-matrix metalloproteinase-9.
• Getting to the heart of asthma: can "beta blockers" be useful to treat asthma?
• Hs04 the use of a visual hand chart to aid in communication of hand injuries.
• Hsp72 induces inflammation and regulates cytokine production in airway epithelium through a TLR4- and NF-kappaB-dependent mechanism.
• Late presentation of congenital bochdalek hernia: a case report.
• Patient response in a screened population for interstitial cystitis.
• Phase I and pharmacokinetic evaluation of the combination of orally administered docetaxel and cyclosporin A in tumor-bearing cats.
• Psychotherapies for hypochondriasis.
• Reduced dosage of pos-1 suppresses Mex mutants and reveals complex interactions among CCCH zinc-finger proteins during Caenorhabditis elegans embryogenesis.
• Regulation of life history determines lifespan of worker honey bees (Apis mellifera L.).
• SIR 2006 annual meeting film panel case: polyarteritis nodosa in upper extremity arteries.
• Shrinking to fit: fluid jettison from a haemocoelic hydrostatic skeleton during defensive withdrawals of a gastropod larva.
• Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost.
• Stroke in former prisoners of war.
• The effect of freezer type, cryoprotectant, and processing methods on viability of frozen embryos.
• The quantitation of bovine embryo viability using a bioluminescent assay for lactate dehydrogenase.
• The relationship of parental overprotection, perceived child vulnerability, and parenting stress to uncertainty in youth with chronic illness.
• Treating systemic effects of COPD.