A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus.

A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus. Research Abstract Details 

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A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus. Abstract Text:

K A Clark,A D Randall,G L Collingridge,

Paired-pulse facilitation of excitatory synaptic transmission was investigated in the CA1 region of rat hippocampal slices using whole-cell patch-clamp recording. To optimise the measurement of excitatory synaptic transmission, gamma-amino-butyric acid (GABA)-mediated synaptic inhibition was eliminated using both GABAA and GABAB antagonists. Pure alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) or N-methyl-D-aspartate (NMDA) receptor-mediated excitatory postsynaptic currents (EPSCs) were then isolated pharmacologically. Paired-pulse facilitation of either AMPA or NMDA receptor-mediated EPSCs (EPSCA and EPSCN, respectively) was investigated using two stimuli of identical strength delivered at intervals of between 25 and 1000 ms. The paired-pulse facilitation profiles of both EPSCA and EPSCN were similar. Paired-pulse facilitation of EPSCA was independent of holding potential. In contrast paired-pulse facilitation of EPSCN was markedly voltage-dependent; maximum facilitation was recorded at hyperpolarised membrane potentials. At positive membrane potentials there was little or no paired-pulse facilitation and, in most neurones, paired-pulse depression was observed. Voltage-dependence of paired-pulse facilitation of EPSCN was similar in the presence of nominal absence of Mg2+ in the bathing medium, and was unaffected by extensive dialysis of neurones with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA). These data are consistent with a presynaptic locus for paired-pulse facilitation of EPSCA. However, paired-pulse facilitation of EPSCN involves postsynaptic factors.

A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus. Publishing Authors By Initials

KA Clark,AD Randall,GL Collingridge,

For similar nervous system: synapses research abstracts see: nervous system: synapses research

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A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental brain research. Experimentelle Hirnfo

VOLUME: 101

Page Numbers: 272-8

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ISSN: 0014-4819

DAY: 15

MONTH: 02

YEAR: 1994

A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus. Information

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LANGUAGE: eng

NlmUniqueID: 43312

A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus. Keywords Mesh Terms:

KEYWORDS: Synapses

MESH TERMS: physiology

Chemical & Substance for Abstract: A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus. Information

Substance Name: Receptors, N-Methyl-D-Aspartate

Registry Number: 0

Grant and Affiliation Information for A comparison of paired-pulsed facilitation of AMPA and NMDA receptor-mediated excitatory postsynaptic currents in the hippocampus.

AFFILIATION: Department of Pharmacology, Medical School, University of Birmingham, Edgbaston, UK.

Country: GERMANY

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MEDLINETA: Exp Brain Res

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