A comparison of mouse and rabbit embryos for the production of transgenic animals by pronuclear microinjection.

A comparison of mouse and rabbit embryos for the production of transgenic animals by pronuclear microinjection. Research Abstract Details 

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A comparison of mouse and rabbit embryos for the production of transgenic animals by pronuclear microinjection. Abstract Text:

A K Voss,A ,G Suske,R M Strojek,M Beato,J Hahn,

Procedures for the production of transgenic animals have low overall efficiency. To evaluate factors responsible for low efficiency, zygotes of two species, varying intensities of microscope light, different qualities of injection pipettes, and six different genes were tested for their influence on the efficiency of pronuclear gene injection for the production of transgenic rabbits and mice. Rabbit zygotes were less sensitive to mechanical manipulation during injection than mouse zygotes. Exposing zygotes to a microscope light intensity of 5550 lx significantly reduced their cleavage rate, while a lower intensity (2280 lx) did not. Using pipettes with a filament for pronuclear gene injection of mouse zygotes resulted in a higher cleavage rate of zygotes after injection than when pipettes were used without filament (30.3 vs 20.6%). Implantation rates varied between 2.9% (HB72CAT) and 23.1% (ts 58-2) depending on the gene used. No transgenic animals were obtained after injection of uteroglobin-CAT-hybrid genes (B2B3UGCAT, HB72CAT), while all other genes used (UG 11.8, UGTAg, RSV lacZ, ts 58-2) resulted in transgenic embryos, fetuses, and newborn animals.

A comparison of mouse and rabbit embryos for the production of transgenic animals by pronuclear microinjection. Publishing Authors By Initials

AK Voss,A ,G Suske,RM Strojek,M Beato,J Hahn,

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A comparison of mouse and rabbit embryos for the production of transgenic animals by pronuclear microinjection. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 34

Page Numbers: 813-24

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Nov

YEAR: 1990

A comparison of mouse and rabbit embryos for the production of transgenic animals by pronuclear microinjection. Information

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LANGUAGE: eng

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Grant and Affiliation Information for A comparison of mouse and rabbit embryos for the production of transgenic animals by pronuclear microinjection.

AFFILIATION: Division of Experimental Reproductive Biology Clinic for Gynecology and Obstetrics School of Veterinary Medicine Hannover Federal Republic of Germany.

Country: United States

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MEDLINETA: Theriogenology

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