A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink.

A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink. Research Abstract Details 

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A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink. Abstract Text:

L E Elsheikh,R Bergman,S J Cryz,B Wretlind,

We have characterized 20 Pseudomonas aeruginosa strains isolated from pneumonic mink lungs with regard to elastase production and serotype. P. aeruginosa PAO1, a well-characterized elastase-producing strain, and two elastase-deficient mutants of PAO1 were used for comparative purposes. Elastase activity was assayed on elastin agar and by using 14C-elastin coated microtiter plates. Elastase antigen was measured using a double antibody sandwich ELISA (enzyme-linked immunosorbent assay). Total proteolytic activity was determined on skim milk agar plates. The results from ELISA showed that all strains produced antigenically similar elastase, although the amounts produced varied considerably between strains. There was a good correlation regarding elastase assays between ELISA and 14C-elastin, elastin agar and total proteolytic activity on skim milk agar. No correlation was found between serotype and elastase activity. The results showed that the 14C-elastin assay is a simple and sensitive method of determining elastolytic activity of P. aeruginosa strains.

A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink. Publishing Authors By Initials

LE Elsheikh,R Bergman,SJ Cryz,B Wretlind,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: pseudomonadaceae: pseudomonas: pseudomonas aeruginosa research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: pseudomonadaceae: pseudomonas: pseudomonas aeruginosa research

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A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta pathologica, microbiologica, et immunologica

VOLUME: 94

Page Numbers: 135-8

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ISSN: 0108-0180

DAY: 12

MONTH: Jun

YEAR: 1986

A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink. Information

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LANGUAGE: eng

NlmUniqueID: 8206623

A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink. Keywords Mesh Terms:

KEYWORDS: Pseudomonas aeruginosa

MESH TERMS: enzymology

Chemical & Substance for Abstract: A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink. Information

Substance Name: Pancreatic Elastase

Registry Number: EC 3.4.21.36

Grant and Affiliation Information for A comparison of different methods for determining elastase activity of Pseudomonas aeruginosa strains from mink.

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Country: DENMARK

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MEDLINETA: Acta Pathol Microbiol Immunol

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