A comparison between methods of identification and serotyping of encapsulated strains of Haemophilus influenzae.

A comparison between methods of identification and serotyping of encapsulated strains of Haemophilus influenzae. Research Abstract Details 

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A comparison between methods of identification and serotyping of encapsulated strains of Haemophilus influenzae. Abstract Text:

I S Taubitz,H Brandis,

Seven methods of serotyping of Haemophilus influenzae were evaluated. Comparing slide agglutination, staphylococcal coagglutination, latex agglutination, counterimmunoelectrophoresis, immunofluorescence, capsular swelling, and cultivation on antiserum agar the commercial coagglutination test was most reliable, most rapid, and easiest to perform. To identify all six serotypes this coagglutination test had to be combined with slide agglutination. With most methods best results were achieved by using cultures incubated at 37 degrees C for 6 h. As nonencapsulated strains often agglutinated unspecifically, selection of probably typeable strains was useful. Differentiation with help of colonial morphology and opalescent growth was facilitated by cultivation on Brain Heart Infusion (BHI) Chocolate Agar and testing of growth factor requirements on translucent BHI Agar with strips containing the growth factors V, X, and VX, respectively. In broth turbid growth was a hint for encapsulation. Nigrosin staining, a negative capsule staining, proved to be useful if specific antisera are not available. From 252 clinical isolates of H. influenzae 216 were not typeable. 36 strains could be serotyped. 27 (75%) belonged to serotype b, 6 (16.6%) were serotype e, 3 (8.3%) were serotype f. Serotype e and f were most difficult to identify. Spectrum of patients and diseases were corresponding to the findings of other authors. Less well-known infections like cellulitis (erysipelas of the cheeks) and arthritis were observed, too. Rapid identification of at least H. influenzae type b could render treatment in some cases more effective by early application of a suitable antibiotic.

A comparison between methods of identification and serotyping of encapsulated strains of Haemophilus influenzae. Publishing Authors By Initials

IS Taubitz,H Brandis,

For similar diagnosis: laboratory techniques and procedures: immunologic tests: serotyping research abstracts see: diagnosis: laboratory techniques and procedures: immunologic tests: serotyping research

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A comparison between methods of identification and serotyping of encapsulated strains of Haemophilus influenzae. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zentralblatt für Bakteriologie, Mikrobiologie, und

VOLUME: 270

Page Numbers: 83-97

Journal Abbreviation:

ISSN: 0176-6724

DAY: 12

MONTH: Nov

YEAR: 1988

A comparison between methods of identification and serotyping of encapsulated strains of Haemophilus influenzae. Information

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LANGUAGE: eng

NlmUniqueID: 8403032

A comparison between methods of identification and serotyping of encapsulated strains of Haemophilus influenzae. Keywords Mesh Terms:

KEYWORDS: Serotyping

MESH TERMS: ultrastructure

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Substance Name: Culture Media

Registry Number: 0

Grant and Affiliation Information for A comparison between methods of identification and serotyping of encapsulated strains of Haemophilus influenzae.

AFFILIATION: Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn.

Country: GERMANY, WEST

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MEDLINETA: Zentralbl Bakteriol Mikrobiol

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